| Literature DB >> 29546734 |
Toru Imai1,2, Junhui Shi1, Terence T W Wong1,2, Lei Li1, Liren Zhu1,2, Lihong V Wang1.
Abstract
Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6 ± 0.2 μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16 min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).Entities:
Keywords: 1-D array ultrasonic transducer; microlens array; photoacoustic imaging; surgical margin assessment; ultraviolet laser
Mesh:
Year: 2018 PMID: 29546734 PMCID: PMC5852316 DOI: 10.1117/1.JBO.23.3.036007
Source DB: PubMed Journal: J Biomed Opt ISSN: 1083-3668 Impact factor: 3.170