Literature DB >> 29546734

High-throughput ultraviolet photoacoustic microscopy with multifocal excitation.

Toru Imai1,2, Junhui Shi1, Terence T W Wong1,2, Lei Li1, Liren Zhu1,2, Lihong V Wang1.   

Abstract

Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6  ±  0.2  μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16  min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

Entities:  

Keywords:  1-D array ultrasonic transducer; microlens array; photoacoustic imaging; surgical margin assessment; ultraviolet laser

Mesh:

Year:  2018        PMID: 29546734      PMCID: PMC5852316          DOI: 10.1117/1.JBO.23.3.036007

Source DB:  PubMed          Journal:  J Biomed Opt        ISSN: 1083-3668            Impact factor:   3.170


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