M Di Sanzo1, R Chirillo1, I Aversa1, F Biamonte1, G Santamaria1, E D Giovannone2, M C Faniello1, G Cuda1, F Costanzo3. 1. Centro di Ricerca di Biochimica e Biologia Molecolare Avanzata, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi "Magna Græcia", Catanzaro, Italy; Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi "Magna Græcia", Catanzaro, Italy. 2. Centro Interdipartimentale di Servizi e Ricerca, Università degli Studi "Magna Græcia", Catanzaro, Italy; Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi "Magna Græcia", Catanzaro, Italy. 3. Centro di Ricerca di Biochimica e Biologia Molecolare Avanzata, Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi "Magna Græcia", Catanzaro, Italy; Dipartimento di Medicina Sperimentale e Clinica, Università degli Studi "Magna Græcia", Catanzaro, Italy. Electronic address: fsc@unicz.it.
Abstract
PURPOSE: The heavy subunit of the iron storage protein ferritin (FHC) is essential for the intracellular iron metabolism and, at the same time, it represents a central hub of iron-independent pathways, such as cell proliferation, angiogenesis, p53 regulation, chemokine signalling, stem cell expansion, miRNAs expression. In this work we have explored the ability of FHC to modulate gene expression in K562 cells, through the up-regulation of the lncRNA H19 and its cognate miR-675. MATERIALS AND METHODS: Targeted silencing of FHC was performed by lentiviral-driven shRNA strategy. FHC reconstitution was obtained by full length FHC cDNA transfection with Lipofectamine 2000. ROS amounts were determined with the redox-sensitive probe H2DCFDA. H19, miR-675, miR-107, Twist1, ID3, EPHB6, GNS, ANK1 and SMAD6 mRNA amounts were quantified by Taqman assay and qPCR analysis. RESULTS: FHC silencing in K562 cells modulates gene expression through the up-regulation of the lncRNA H19 and its cognate miR-675. Experimental findings demonstrate that the molecular mechanism underlying this phenomenon is represented by an FHC knock-down-triggered increase in reactive oxygen species (ROS) production. CONCLUSIONS: In this paper we uncover a so far not described function of the ferritin heavy subunit in the control of lncRNA pathways.
PURPOSE: The heavy subunit of the iron storage protein ferritin (FHC) is essential for the intracellular iron metabolism and, at the same time, it represents a central hub of iron-independent pathways, such as cell proliferation, angiogenesis, p53 regulation, chemokine signalling, stem cell expansion, miRNAs expression. In this work we have explored the ability of FHC to modulate gene expression in K562 cells, through the up-regulation of the lncRNA H19 and its cognate miR-675. MATERIALS AND METHODS: Targeted silencing of FHC was performed by lentiviral-driven shRNA strategy. FHC reconstitution was obtained by full length FHC cDNA transfection with Lipofectamine 2000. ROS amounts were determined with the redox-sensitive probe H2DCFDA. H19, miR-675, miR-107, Twist1, ID3, EPHB6, GNS, ANK1 and SMAD6 mRNA amounts were quantified by Taqman assay and qPCR analysis. RESULTS:FHC silencing in K562 cells modulates gene expression through the up-regulation of the lncRNA H19 and its cognate miR-675. Experimental findings demonstrate that the molecular mechanism underlying this phenomenon is represented by an FHC knock-down-triggered increase in reactive oxygen species (ROS) production. CONCLUSIONS: In this paper we uncover a so far not described function of the ferritin heavy subunit in the control of lncRNA pathways.
Authors: Anna Martina Battaglia; Alessandro Sacco; Ida Daniela Perrotta; Maria Concetta Faniello; Mariangela Scalise; Daniele Torella; Sonia Levi; Francesco Costanzo; Flavia Biamonte Journal: Front Oncol Date: 2022-03-31 Impact factor: 6.244
Authors: Roberta Chirillo; Ilenia Aversa; Anna Di Vito; Alessandro Salatino; Anna Martina Battaglia; Alessandro Sacco; Maddalena Adriana Di Sanzo; Maria Concetta Faniello; Barbara Quaresima; Camillo Palmieri; Flavia Biamonte; Francesco Costanzo Journal: Front Oncol Date: 2020-05-05 Impact factor: 6.244
Authors: Alessandro Salatino; Ilenia Aversa; Anna Martina Battaglia; Alessandro Sacco; Anna Di Vito; Gianluca Santamaria; Roberta Chirillo; Pierangelo Veltri; Giuseppe Tradigo; Annalisa Di Cello; Roberta Venturella; Flavia Biamonte; Francesco Costanzo Journal: Oxid Med Cell Longev Date: 2019-10-31 Impact factor: 6.543