Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.

Literature DB >> 29542839

Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.

Teresa Cruz-Bustos¹, Silvia N J Moreno^1,2, Roberto Docampo^1,2.

Abstract

Membrane proteins in trypanosomatids are, in general, weakly expressed and confirmation of their subcellular localization frequently requires their overexpression with epitope tags. However, overexpression can lead to mislocalization of the probes. Viswanathan et al. (Nat. Methods, 2015, 12:568) described high performance tags for localization of weakly expressed proteins. We report here the use of these protein tags, named "spaghetti monster," for CRISPR/Cas9-mediated C-terminal endogenous tagging of Trypanosoma cruzi to localize two weakly expressed transient receptor potential channels to acidic compartments. The results indicate that this method will improve the detection of membrane proteins in T. cruzi.

Entities: Chemical Disease Gene Species

Keywords: zzm321990Trypanosoma cruzizzm321990; Acidic compartmens; gene tagging; lysosomes; spaghetti monster tag

Mesh：

Substances：

Year: 2018 PMID： 29542839 PMCID： PMC6120783 DOI： 10.1111/jeu.12517

Source DB: PubMed Journal: J Eukaryot Microbiol ISSN： 1066-5234 Impact factor: 3.346

28 in total

1. Isotopes incorporated in the nucleic acids of Trypanosoma mega.

Authors: G J BONE; M STEINERT
Journal: Nature Date: 1956-08-11 Impact factor: 49.962

2. A combined transmembrane topology and signal peptide prediction method.

Authors: Lukas Käll; Anders Krogh; Erik L L Sonnhammer
Journal: J Mol Biol Date: 2004-05-14 Impact factor: 5.469

Review 3. TRP channels.

Authors: Kartik Venkatachalam; Craig Montell
Journal: Annu Rev Biochem Date: 2007 Impact factor: 23.643

4. A new bioinformatics analysis tools framework at EMBL-EBI.

Authors: Mickael Goujon; Hamish McWilliam; Weizhong Li; Franck Valentin; Silvano Squizzato; Juri Paern; Rodrigo Lopez
Journal: Nucleic Acids Res Date: 2010-05-03 Impact factor: 16.971

5. Identification of intracellular and plasma membrane calcium channel homologues in pathogenic parasites.

Authors: David L Prole; Colin W Taylor
Journal: PLoS One Date: 2011-10-14 Impact factor: 3.240

6. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.

Authors: Fabian Sievers; Andreas Wilm; David Dineen; Toby J Gibson; Kevin Karplus; Weizhong Li; Rodrigo Lopez; Hamish McWilliam; Michael Remmert; Johannes Söding; Julie D Thompson; Desmond G Higgins
Journal: Mol Syst Biol Date: 2011-10-11 Impact factor: 11.429

7. InterProScan 5: genome-scale protein function classification.

Authors: Philip Jones; David Binns; Hsin-Yu Chang; Matthew Fraser; Weizhong Li; Craig McAnulla; Hamish McWilliam; John Maslen; Alex Mitchell; Gift Nuka; Sebastien Pesseat; Antony F Quinn; Amaia Sangrador-Vegas; Maxim Scheremetjew; Siew-Yit Yong; Rodrigo Lopez; Sarah Hunter
Journal: Bioinformatics Date: 2014-01-21 Impact factor: 6.937

8. High-performance probes for light and electron microscopy.

Authors: Sarada Viswanathan; Megan E Williams; Erik B Bloss; Timothy J Stasevich; Colenso M Speer; Aljoscha Nern; Barret D Pfeiffer; Bryan M Hooks; Wei-Ping Li; Brian P English; Teresa Tian; Gilbert L Henry; John J Macklin; Ronak Patel; Charles R Gerfen; Xiaowei Zhuang; Yalin Wang; Gerald M Rubin; Loren L Looger
Journal: Nat Methods Date: 2015-04-27 Impact factor: 28.547

9. The Pfam protein families database: towards a more sustainable future.

Authors: Robert D Finn; Penelope Coggill; Ruth Y Eberhardt; Sean R Eddy; Jaina Mistry; Alex L Mitchell; Simon C Potter; Marco Punta; Matloob Qureshi; Amaia Sangrador-Vegas; Gustavo A Salazar; John Tate; Alex Bateman
Journal: Nucleic Acids Res Date: 2015-12-15 Impact factor: 16.971

4. The Mitochondrial Ca²⁺ Uniporter Complex (MCUC) of Trypanosoma brucei Is a Hetero-oligomer That Contains Novel Subunits Essential for Ca²⁺ Uptake.

Authors: Guozhong Huang; Roberto Docampo
Journal: MBio Date: 2018-09-18 Impact factor: 7.867

4 in total

Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High-Performance Probes.

1. Isotopes incorporated in the nucleic acids of Trypanosoma mega.

2. A combined transmembrane topology and signal peptide prediction method.

Review 3. TRP channels.

4. A new bioinformatics analysis tools framework at EMBL-EBI.

5. Identification of intracellular and plasma membrane calcium channel homologues in pathogenic parasites.

6. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.

7. InterProScan 5: genome-scale protein function classification.

8. High-performance probes for light and electron microscopy.

9. The Pfam protein families database: towards a more sustainable future.

10. Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins.

1. Tagging of Weakly Expressed Toxoplasma gondii Calcium-Related Genes with High-Affinity Tags.

Review 2. State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids.

Review 3. Membrane Proteins in Trypanosomatids Involved in Ca²⁺ Homeostasis and Signaling.

4. The Mitochondrial Ca²⁺ Uniporter Complex (MCUC) of Trypanosoma brucei Is a Hetero-oligomer That Contains Novel Subunits Essential for Ca²⁺ Uptake.

Review 3. TRP channels.

Review 2. State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids.

Review 3. Membrane Proteins in Trypanosomatids Involved in Ca2+ Homeostasis and Signaling.

Review 3. Membrane Proteins in Trypanosomatids Involved in Ca²⁺ Homeostasis and Signaling.