Chen Chen1, Wenzhong Hu1, Ruidong Zhang2, Aili Jiang1, Chenghui Liu1. 1. Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, China. 2. Dayaowan Entry-Exit Inspection and Quarantine Bureau, Dalian, China.
Abstract
BACKGROUND: The effect of hydrogen sulfide (H2 S) on the inhibition of the surface whitening of fresh-cut carrots was investigated. Whitening index; H2 O2 and malondialdehyde (MDA) contents; activities of catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD); and antioxidant capacities were evaluated. RESULTS: H2 S treatment significantly inhibited the surface whitening of fresh-cut carrots (P < 0.05), reduced the accumulation of H2 O2 and MDA, and enhanced antioxidant enzymes (CAT, APX and GR) activities and antioxidant capacities (P < 0.05). The PAL, PPO and POD activities of fresh-cut carrots were inhibited by treatment with H2 S (P < 0.05). Furthermore, correlation analysis revealed that whitening index was significantly positively correlated with H2 O2 content, lipid peroxidation, PPO and POD activities. CONCLUSION: H2 S inhibited the surface whitening of fresh-cut carrots by reducing H2 O2 accumulation and lipid peroxidation, and also inhibited PPO and POD activities.
BACKGROUND: The effect of hydrogen sulfide (H2 S) on the inhibition of the surface whitening of fresh-cut carrots was investigated. Whitening index; H2 O2 and malondialdehyde (MDA) contents; activities of catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD); and antioxidant capacities were evaluated. RESULTS:H2 S treatment significantly inhibited the surface whitening of fresh-cut carrots (P < 0.05), reduced the accumulation of H2 O2 and MDA, and enhanced antioxidant enzymes (CAT, APX and GR) activities and antioxidant capacities (P < 0.05). The PAL, PPO and POD activities of fresh-cut carrots were inhibited by treatment with H2 S (P < 0.05). Furthermore, correlation analysis revealed that whitening index was significantly positively correlated with H2 O2 content, lipid peroxidation, PPO and POD activities. CONCLUSION:H2 S inhibited the surface whitening of fresh-cut carrots by reducing H2 O2 accumulation and lipid peroxidation, and also inhibited PPO and POD activities.