Literature DB >> 29541676

Deformed Wing virus absence/presence data across three genera on two Hawaiian Islands.

Abstract

The data presented in this article relates to the research article, "Evidence of Varroa-mediated Deformed Wing virus spillover in Hawaii" (Santamaria et al., 2017) [3]. The article presents data collected throughout August 2014 to November 2015, on the two Hawaiian Islands of Oahu and Maui. Apis and non-Apis specimens - a total of four species - were collected and tested for Deformed Wing virus (DWV) absence or presence, only. Specific island locations are noted. This data is made publicly available to be analyzed or used in future relevant research.

Entities: Disease Species

Keywords: Apis mellifera; Ceratina smaragdula; Deformed Wing virus; Hawaii; Polistes; pollinator

Year: 2017 PMID： 29541676 PMCID： PMC5847618 DOI： 10.1016/j.dib.2017.12.020

Source DB: PubMed Journal: Data Brief ISSN： 2352-3409

Specifications Table Value of the data Data is useful as benchmark for viral presence in a pre-Varroa ecosystem Data implicates how important it is to keep Varroa out of the Varroa-free islands of Hawaii Potential future studies can explore pollinator-flower webs and how viruses could spread in these systems

Data

The data in this article lists which species were collected, from which island, and island location they were collected from. The data shows whether each sample tested positive for Deformed Wing virus (DWV) or not; this is represented by “0” and “1” to indicate absence or presence, respectively. The raw data can be seen in Table 1.

Table 1

Four species across three different genera were collected on the Hawaiian Islands of Oahu and/or Maui. Specific island location is noted. Deformed Wing virus (DWV) absence/presence is indicated by “0” or “1” respectively.

DWV	Species	Island	Location
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Kahului
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Haleakala
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
0	Apis mellifera	Maui	Iao Needle
1	Apis mellifera	Maui	Kahului
1	Apis mellifera	Maui	Iao Needle
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Ceratina smaragdula	Maui	Kahului
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Polistes exclamans	Maui	Makawao
0	Apis mellifera	Oahu	Ho'o Maluhia
0	Apis mellifera	Oahu	Ho'o Maluhia
0	Apis mellifera	Oahu	Ho'o Maluhia
0	Apis mellifera	Oahu	Ho'o Maluhia
0	Apis mellifera	Oahu	Sandy Beach
0	Apis mellifera	Oahu	Sandy Beach
0	Apis mellifera	Oahu	Sandy Beach
0	Apis mellifera	Oahu	Sandy Beach
0	Apis mellifera	Oahu	Sandy Beach
0	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Ho'o Maluhia
1	Apis mellifera	Oahu	Ho'o Maluhia
1	Apis mellifera	Oahu	Ho'o Maluhia
1	Apis mellifera	Oahu	Ho'o Maluhia
1	Apis mellifera	Oahu	Ho'o Maluhia
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Manoa
1	Apis mellifera	Oahu	Manoa
1	Apis mellifera	Oahu	Manoa
1	Apis mellifera	Oahu	Manoa
1	Apis mellifera	Oahu	Manoa
1	Apis mellifera	Oahu	Sandy Beach
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
1	Apis mellifera	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Ho'o Maluhia
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Manoa
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Sandy Beach
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
0	Ceratina smaragdula	Oahu	Pearl City
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Kaena Point
1	Ceratina smaragdula	Oahu	Pearl City
1	Ceratina smaragdula	Oahu	Pearl City
1	Ceratina smaragdula	Oahu	Pearl City
1	Ceratina smaragdula	Oahu	Ho'o Maluhia
1	Ceratina smaragdula	Oahu	Ho'o Maluhia
1	Ceratina smaragdula	Oahu	Ho'o Maluhia
1	Ceratina smaragdula	Oahu	Manoa
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
0	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo
1	Polistes aurifer	Oahu	Waimanalo

Experimental design, materials and methods

Specimen collection

Three species within two different Hymenoptera genera were selected as representatives of the local community of flower visitors: the introduced small carpenter bee Ceratina smaragdula (Apidae) which was first recorded in Hawaii in 1999 [2], and the introduced paper wasps Polistes aurifer and Polistes exclamans (Vespidae) first recorded in Hawaii in the 19th century and in 1952 respectively [1]. Polistes wasps collected on Oahu are P. aurifer and the specimens from Maui are P. exclamans. All samples were collected from either of the five sites on Oahu (Varroa-positive island), or the four sites on Maui, (Varroa-negative island). Collection sites on both islands consisted on a mix of agricultural fields, parks, gardens, and beach edge vegetation strips. The selected insect species are all relatively abundant and can be found in urban and agricultural environments, where they overlap in resource use with A. mellifera. Samples were collected from August 2014 to November 2015. Insects were collected while they were foraging in fields or flower patches, via a hand-held net. Paper wasp samples were also collected from around their nests. Each insect was stored individually and kept on ice in the field until transferred to a -80 °C freezer for long term storage.

RNA extraction and reverse transcription PCR

Methods for RNA extraction and RT-PCR are fully described in Santamaria et al. [3].

Subject area	Entomology, Virology
More specific subject area	Insect virology
Type of data	Table
How data was acquired	A stained SYBR Safe DNA gel stain (Invitrogen) was visualized under ultraviolet light. The positive samples were noted with “1” and negative samples with “0”.
Data format	Raw
Experimental factors	Samples were either from Oahu or Maui
Experimental features	Samples were collected on the two different islands, Oahu or Maui, across different sites. Samples were preserved in −80 °C until they were tested for absence or presence of the Deformed Wing virus.
Data source location	Kahului, Hawaii
	Haleakala, Hawaii
	Iao Needle, Hawaii
	Makawao, Hawaii
	Ho’omaluhia, Hawaii
	Sandy Beach, Hawaii
	Pearl City, Hawaii
	Manoa, Hawaii
	Kaena Point, Hawaii
	Waimanalo, Hawaii
Data accessibility	Data is within this article

1 in total

1. Evidence of Varroa-mediated deformed wing virus spillover in Hawaii.

Authors: Jessika Santamaria; Ethel M Villalobos; Laura E Brettell; Scott Nikaido; Jason R Graham; Stephen Martin
Journal: J Invertebr Pathol Date: 2017-11-21 Impact factor: 2.841

1 in total