C M S Figueredo 1 , M S M Ribeiro 2 , R G Fischer 2 , A Gustafsson 1 Show Affiliations »
Abstract
BACKGROUND: The levels of interleukin-1β (IL-1β) have been reported to be higher in sites with periodontitis than in healthy controls. This may be the result of a more severe inflammation and/or constitutional differences in IL-1β production. Our aim was to test the hypothesis that the level of IL-1β in gingival crevicular fluid (GCF) is a characteristic trait of periodontitis, regardless of the degree of tissue destruction. As a secondary aim, we investigated the correlation between IL-1β and neutrophil elastase. An untreated population was used. METHODS: GCF samples from inflamed sites in 13 patients with gingivitis (GG), and from inflamed sites with shallow (GP) and deep (PP) pockets in 18 patients with periodontitis were collected. Interleukin-1β, elastase-α-1-antitrypsin complex (E-A1AT), α-1-antitrypsin (A1AT), and α-2-macroglobulin (A2MG) were measured with ELISA. Elastase activity was measured with a specific substrate. RESULTS: The concentration of IL-1β was significantly higher in PP than in GG, but no significant differences were detected between PP and GP. A weak positive correlation between the elastase activity and IL-1β was also observed. The elastase activity had a tendency to be higher in PP, although no significant difference was found among the 3 groups. The concentrations of E-A1AT, A1AT, and A2MG were similar in the 3 types of sites. CONCLUSIONS: The levels of IL-1β in GCF were increased in samples from periodontitis patients, regardless of the severity of disease at the sampled site, suggesting that the levels of IL-1β are typical of a given patient. J Periodontol 1999;70:1457-1463. © 1999 American Academy of Periodontology.
BACKGROUND: The levels of interleukin-1β (IL-1β) have been reported to be higher in sites with periodontitis than in healthy controls. This may be the result of a more severe inflammation and/or constitutional differences in IL-1β production. Our aim was to test the hypothesis that the level of IL-1β in gingival crevicular fluid (GCF) is a characteristic trait of periodontitis , regardless of the degree of tissue destruction. As a secondary aim, we investigated the correlation between IL-1β and neutrophil elastase . An untreated population was used. METHODS: GCF samples from inflamed sites in 13 patients with gingivitis (GG), and from inflamed sites with shallow (GP) and deep (PP) pockets in 18 patients with periodontitis were collected. Interleukin-1β, elastase -α-1-antitrypsin complex (E-A1AT ), α-1-antitrypsin (A1AT ), and α-2-macroglobulin (A2MG) were measured with ELISA. Elastase activity was measured with a specific substrate. RESULTS: The concentration of IL-1β was significantly higher in PP than in GG, but no significant differences were detected between PP and GP. A weak positive correlation between the elastase activity and IL-1β was also observed. The elastase activity had a tendency to be higher in PP, although no significant difference was found among the 3 groups. The concentrations of E-A1AT , A1AT , and A2MG were similar in the 3 types of sites. CONCLUSIONS: The levels of IL-1β in GCF were increased in samples from periodontitis patients , regardless of the severity of disease at the sampled site, suggesting that the levels of IL-1β are typical of a given patient . J Periodontol 1999;70:1457-1463. © 1999 American Academy of Periodontology.
Entities: Disease
Gene
Species
Keywords:
Gingival crevicular fluid/analysis; gingivitis; interleukin-1; neutrophils; periodontitis
Year: 1999
PMID： 29539870 DOI： 10.1902/jop.1999.70.12.1457
Source DB: PubMed Journal: J Periodontol ISSN： 0022-3492 Impact factor: 6.993