Yen-Ying Chen1,2, Hsiang-Ling Ho1,2, Shih-Chieh Lin1,2, Tiffany Dai-Hwa Ho3, Chih-Yi Hsu1,2,4. 1. Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. 2. School of Medicine, National Yang-Ming University, Taipei, Taiwan. 3. Departments of Computer Science and Statistics, Duke University, Durham, NC, Taiwan. 4. College of Nursing, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan.
Abstract
OBJECTIVES: To evaluate the prognostic values of microRNAs (miRNAs) in glioblastoma, and to see if there is an association between miRNAs and MGMT promoter methylation status. METHODS: We collected paraffin blocks from resection specimens from 114 glioblastoma patients who had received temozolomide treatment and radiotherapy. Real-time quantitative PCR was performed to determine the expression levels of five miRNAs. RESULTS: Upregulation of miR 125b-5p, miR 181d-3p, miR 221-3p, miR-222-3p, and miR 224-5p was observed in 13.2%, 5.3%, 12.3%, 32.5%, and 78.9% of the cases, respectively. The expression level of miRNAs was not significantly different in tumors with MGMT promoter methylation vs tumors without such methylation. Upregulation of miR 125b-5p, miR 181d-3p, or miR 221-3p was significantly associated with shorter survival in MGMT-unmethylated glioblastoma patients. CONCLUSIONS: miR 125b-5p, miR 181d-3p, and miR 221-3p are useful in predicting poor prognosis in patients with MGMT-unmethylated glioblastomas.
OBJECTIVES: To evaluate the prognostic values of microRNAs (miRNAs) in glioblastoma, and to see if there is an association between miRNAs and MGMT promoter methylation status. METHODS: We collected paraffin blocks from resection specimens from 114 glioblastoma patients who had received temozolomide treatment and radiotherapy. Real-time quantitative PCR was performed to determine the expression levels of five miRNAs. RESULTS: Upregulation of miR 125b-5p, miR 181d-3p, miR 221-3p, miR-222-3p, and miR 224-5p was observed in 13.2%, 5.3%, 12.3%, 32.5%, and 78.9% of the cases, respectively. The expression level of miRNAs was not significantly different in tumors with MGMT promoter methylation vs tumors without such methylation. Upregulation of miR 125b-5p, miR 181d-3p, or miR 221-3p was significantly associated with shorter survival in MGMT-unmethylated glioblastoma patients. CONCLUSIONS: miR 125b-5p, miR 181d-3p, and miR 221-3p are useful in predicting poor prognosis in patients with MGMT-unmethylated glioblastomas.
Authors: Artemiy S Silantyev; Luca Falzone; Massimo Libra; Olga I Gurina; Karina Sh Kardashova; Taxiarchis K Nikolouzakis; Alexander E Nosyrev; Christopher W Sutton; Panayiotis D. Mitsias; Aristides Tsatsakis Journal: Cells Date: 2019-08-09 Impact factor: 6.600
Authors: Elena Schnabel; Maximilian Knoll; Christian Schwager; Rolf Warta; Andreas Mock; Benito Campos; Laila König; Christine Jungk; Wolfgang Wick; Andreas Unterberg; Jürgen Debus; Christel Herold-Mende; Amir Abdollahi Journal: Int J Mol Sci Date: 2021-03-15 Impact factor: 5.923