Adam J Krieg1,2,3,4,5, Sarah R Mullinax6, Frances Grimstad2, Kaitlin Marquis2, Elizabeth Constance2, Yan Hong2, Sacha A Krieg1,7,2,5, Katherine F Roby8,9,10. 1. Institute for Reproductive Health and Regenerative Medicine, Center for Reproductive Sciences, University of Kansas Medical Center, 3901 Rainbow Blvd. MS3050, Kansas City, KS, 66160, USA. 2. Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, KS, 66160, USA. 3. Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA. 4. Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR, USA. 5. Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, 97006, USA. 6. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA. 7. Women's Health Specialty Center Advanced Reproductive Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA. 8. Institute for Reproductive Health and Regenerative Medicine, Center for Reproductive Sciences, University of Kansas Medical Center, 3901 Rainbow Blvd. MS3050, Kansas City, KS, 66160, USA. kroby@kumc.edu. 9. Women's Health Specialty Center Advanced Reproductive Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA. kroby@kumc.edu. 10. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS, 66160, USA. kroby@kumc.edu.
Abstract
PURPOSE: To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome. METHODS: Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens. RESULTS: KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group. CONCLUSIONS: Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.
PURPOSE: To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome. METHODS: Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens. RESULTS:KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group. CONCLUSIONS: Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.
Authors: Meredith P Provost; Kelly S Acharya; Chaitanya R Acharya; Jason S Yeh; Ryan G Steward; Jennifer L Eaton; James M Goldfarb; Suheil J Muasher Journal: Fertil Steril Date: 2015-11-25 Impact factor: 7.329
Authors: Joshua C Black; Elnaz Atabakhsh; Jaegil Kim; Kelly M Biette; Capucine Van Rechem; Brendon Ladd; Paul D Burrowes; Carlos Donado; Hamid Mattoo; Benjamin P Kleinstiver; Bing Song; Grasiella Andriani; J Keith Joung; Othon Iliopoulos; Cristina Montagna; Shiv Pillai; Gad Getz; Johnathan R Whetstine Journal: Genes Dev Date: 2015-05-15 Impact factor: 11.361