Bunpei Katayama1, Toshiyuki Ozawa2, Kuniyuki Morimoto1, Kunio Awazu3, Nobuhisa Ito3, Norihiro Honda4, Naoki Oiso5, Daisuke Tsuruta6. 1. Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan. 2. Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan; Research Center for Infectious Disease Sciences, Osaka City University Graduate School of Medicine, Osaka, Japan. Electronic address: ozawa@med.osaka-cu.ac.jp. 3. Medical Beam Physics Laboratory, Osaka University Graduate School of Engineering, Osaka, Japan. 4. Medical Beam Physics Laboratory, Osaka University Graduate School of Engineering, Osaka, Japan; Institute for Academic Initiatives, Osaka University, Osaka, Japan. 5. Department of Dermatology, Kindai University Faculty of Medicine, Osaka, Japan. 6. Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan; Research Center for Infectious Disease Sciences, Osaka City University Graduate School of Medicine, Osaka, Japan.
Abstract
BACKGROUND: Pseudomonas aeruginosa (PA) frequently develops antibiotic-resistant characteristics, which is clinically problematic. The main reason behind the rise of antibiotic-resistant PA is the extensive use of antibiotics. Therefore, a novel technique is needed to treat PA infections. Photodynamic therapy (PDT) is thought to have the potential to be a non-antibiotic treatment for infections. 5-Aminolevulinic acid (ALA), which works as a photosensitizer after being metabolized into protoporphyrin IX (PpIX) in the heme synthetic pathway, is used for PDT. Thus far, the in vivo effectiveness of PDT using ALA against PA is unknown. OBJECTIVE: In this study, we investigated PDT using ALA both in vitro and in vivo. METHODS AND RESULTS: Although PDT with ALA alone did not show a bactericidal effect on PA, PDT with both ALA and EDTA-2Na had a bactericidal effect in vitro. In in vivo experiments, wounds healed faster in PA-infected mice treated with PDT using both EDTA-2Na and ALA compared to non-PDT. CONCLUSION: These results suggest that PDT with EDTA-2Na and ALA is a potential novel treatment option for PA-infected wounds.
BACKGROUND:Pseudomonas aeruginosa (PA) frequently develops antibiotic-resistant characteristics, which is clinically problematic. The main reason behind the rise of antibiotic-resistant PA is the extensive use of antibiotics. Therefore, a novel technique is needed to treat PA infections. Photodynamic therapy (PDT) is thought to have the potential to be a non-antibiotic treatment for infections. 5-Aminolevulinic acid (ALA), which works as a photosensitizer after being metabolized into protoporphyrin IX (PpIX) in the heme synthetic pathway, is used for PDT. Thus far, the in vivo effectiveness of PDT using ALA against PA is unknown. OBJECTIVE: In this study, we investigated PDT using ALA both in vitro and in vivo. METHODS AND RESULTS: Although PDT with ALA alone did not show a bactericidal effect on PA, PDT with both ALA and EDTA-2Na had a bactericidal effect in vitro. In in vivo experiments, wounds healed faster in PA-infectedmice treated with PDT using both EDTA-2Na and ALA compared to non-PDT. CONCLUSION: These results suggest that PDT with EDTA-2Na and ALA is a potential novel treatment option for PA-infected wounds.
Authors: M R Tuttobene; J F Pérez; E S Pavesi; B Perez Mora; D Biancotti; P Cribb; M Altilio; G L Müller; H Gramajo; G Tamagno; M S Ramírez; L Diacovich; M A Mussi Journal: J Bacteriol Date: 2021-02-08 Impact factor: 3.490