Zhaohua Shen1,2, Changxin Zhu1,2, Yongsheng Quan1,2, Jinming Yang3, Wei Yuan1,2, Zhenyu Yang1,2, Shuai Wu1,2, Weiwei Luo1,2, Bei Tan1,2, Xiaoyan Wang1,2. 1. Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, Hunan, China. 2. Hunan Key Laboratory of Non-resolving Inflammation and Cancer, Changsha, Hunan, China. 3. Department of Pharmacology and The Penn State Cancer Institute, The Pennsylvania State University College of Medicine, and Milton S Hershey Medical Center, Hershey, Pennsylvania, USA.
Abstract
BACKGROUND AND AIM: The study aims to elucidate the anti-inflammatory effect and mechanism of Roseburia intestinalis (R. intestinalis) in Crohn's disease (CD). METHODS: 16S-rRNA genome sequencing technique is used to detect the characteristics of intestinal microbiota in untreated CD patients and healthy controls. Then the study investigates the effects of R. intestinalis on disease activity index score, intestinal pathology, the differentiation of Treg cells, and the expressions of Thymic stromal lymphopoietin (TSLP), TGF-β and IL-10 by using TNBS colitis models. At the cellular level, the study uses LPS to stimulate Caco-2 cells to conduct inflammation models and then co-culture with R. intestinalis and detect changes of TSLP and TGF-β. The study then uses R. intestinalis to stimulate peripheral blood mononuclear cells, and the change of Treg cells was detected. RESULTS: Genome sequencing of fecal samples from untreated CD patients (n = 10) revealed decreases in the abundance and diversity of intestinal microbiota, including R. intestinalis. Moreover, R. intestinalis reduced disease activity index scores, colon shortening, intestinal mucosal epithelial injury, and mucosal lymphocyte infiltration in a colitis mice model. It suppressed intestinal inflammation by increasing Treg cell numbers and expression of the anti-inflammatory cytokines TSLP, TGF-β, and interleukin-10 (P < 0.05). R. intestinalis also increased secretion of TSLP and TGF-β in lipopolysaccharide-treated Caco-2 cells. CONCLUSION: These findings suggest that R. intestinalis suppresses CD pathogenesis by inducing anti-inflammatory responses.
BACKGROUND AND AIM: The study aims to elucidate the anti-inflammatory effect and mechanism of Roseburia intestinalis (R. intestinalis) in Crohn's disease (CD). METHODS: 16S-rRNA genome sequencing technique is used to detect the characteristics of intestinal microbiota in untreated CDpatients and healthy controls. Then the study investigates the effects of R. intestinalis on disease activity index score, intestinal pathology, the differentiation of Treg cells, and the expressions of Thymic stromal lymphopoietin (TSLP), TGF-β and IL-10 by using TNBS colitis models. At the cellular level, the study uses LPS to stimulate Caco-2 cells to conduct inflammation models and then co-culture with R. intestinalis and detect changes of TSLP and TGF-β. The study then uses R. intestinalis to stimulate peripheral blood mononuclear cells, and the change of Treg cells was detected. RESULTS: Genome sequencing of fecal samples from untreated CDpatients (n = 10) revealed decreases in the abundance and diversity of intestinal microbiota, including R. intestinalis. Moreover, R. intestinalis reduced disease activity index scores, colon shortening, intestinal mucosal epithelial injury, and mucosal lymphocyte infiltration in a colitismice model. It suppressed intestinal inflammation by increasing Treg cell numbers and expression of the anti-inflammatory cytokines TSLP, TGF-β, and interleukin-10 (P < 0.05). R. intestinalis also increased secretion of TSLP and TGF-β in lipopolysaccharide-treated Caco-2 cells. CONCLUSION: These findings suggest that R. intestinalis suppresses CD pathogenesis by inducing anti-inflammatory responses.
Authors: Aubrey M Schonhoff; Gregory P Williams; Zachary D Wallen; David G Standaert; Ashley S Harms Journal: Prog Brain Res Date: 2019-12-05 Impact factor: 2.453
Authors: Ruth Rattigan; John V O'Doherty; Stafford Vigors; Marion T Ryan; Rocco S Sebastiano; John J Callanan; Kevin Thornton; Gaurav Rajauria; Lekha M Margassery; Alan D W Dobson; Niall D O'Leary; Torres Sweeney Journal: Mar Drugs Date: 2020-05-16 Impact factor: 5.118
Authors: Yun Fei Wang; Li Juan Zheng; Ya Liu; Yu Bing Ye; Song Luo; Guang Ming Lu; Dehua Gong; Long Jiang Zhang Journal: Theranostics Date: 2019-10-18 Impact factor: 11.556