| Literature DB >> 2953134 |
Abstract
PAPS-reductase from Escherichia coli was employed to detect thioredoxins from pro- and eukaryotic organisms. A simple method for the isolation of this enzyme and properties of the enzymatic assay were described. A comparison between thioredoxins detected by the PAPS-reductase and the Fructose-bisphosphatase or NADP malate dehydrogenase was used to assess the validity of the test. The high cross-reactivity of the bacterial enzyme was useful in the purification of heterologous thioredoxins from spinach, Synechococcus, and Saccharomyces cerevisiae.Entities:
Mesh:
Substances:
Year: 1987 PMID: 2953134 DOI: 10.1515/znc-1987-1-216
Source DB: PubMed Journal: Z Naturforsch C J Biosci ISSN: 0341-0382