Literature DB >> 2953134

PAPS-reductase from Escherichia coli: characterization of the enzyme as probe for thioredoxins.

J D Schwenn, U Schriek.   

Abstract

PAPS-reductase from Escherichia coli was employed to detect thioredoxins from pro- and eukaryotic organisms. A simple method for the isolation of this enzyme and properties of the enzymatic assay were described. A comparison between thioredoxins detected by the PAPS-reductase and the Fructose-bisphosphatase or NADP malate dehydrogenase was used to assess the validity of the test. The high cross-reactivity of the bacterial enzyme was useful in the purification of heterologous thioredoxins from spinach, Synechococcus, and Saccharomyces cerevisiae.

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Year:  1987        PMID: 2953134     DOI: 10.1515/znc-1987-1-216

Source DB:  PubMed          Journal:  Z Naturforsch C J Biosci        ISSN: 0341-0382


  10 in total

1.  Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli.

Authors:  F A Krone; G Westphal; J D Schwenn
Journal:  Mol Gen Genet       Date:  1991-02

Review 2.  The role of 5'-adenylylsulfate reductase in controlling sulfate reduction in plants.

Authors:  Melinda N Martin; Mitchell C Tarczynski; Bo Shen; Thomas Leustek
Journal:  Photosynth Res       Date:  2005-11-15       Impact factor: 3.573

3.  Yeast PAPS reductase: properties and requirements of the purified enzyme.

Authors:  J D Schwenn; F A Krone; K Husmann
Journal:  Arch Microbiol       Date:  1988       Impact factor: 2.552

4.  Selection and characterization of sethoxydim- tolerant maize tissue cultures.

Authors:  W B Parker; D A Somers; D L Wyse; R A Keith; J D Burton; J W Gronwald; B G Gengenbach
Journal:  Plant Physiol       Date:  1990-04       Impact factor: 8.340

5.  Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity.

Authors:  J F Gutierrez-Marcos; M A Roberts; E I Campbell; J L Wray
Journal:  Proc Natl Acad Sci U S A       Date:  1996-11-12       Impact factor: 11.205

6.  Effects of Acetyl-Coenzyme A Carboxylase Inhibitors on Root Cell Transmembrane Electric Potentials in Graminicide-Tolerant and -Susceptible Corn (Zea mays L.).

Authors:  P. A. Dotray; J. M. DiTomaso; J. W. Gronwald; D. L. Wyse; L. V. Kochian
Journal:  Plant Physiol       Date:  1993-11       Impact factor: 8.340

7.  Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases.

Authors:  Md Faruq Hossain; Yana Bodnar; Calvin Klein; Clara Ortegón Salas; Elias S J Arnér; Manuela Gellert; Christopher Horst Lillig
Journal:  Oxid Med Cell Longev       Date:  2021-06-01       Impact factor: 6.543

8.  Co-expression of bacterial aspartate kinase and adenylylsulfate reductase genes substantially increases sulfur amino acid levels in transgenic alfalfa (Medicago sativa L.).

Authors:  Zongyong Tong; Can Xie; Lei Ma; Liping Liu; Yongsheng Jin; Jiangli Dong; Tao Wang
Journal:  PLoS One       Date:  2014-02-10       Impact factor: 3.240

9.  The specificity of thioredoxins and glutaredoxins is determined by electrostatic and geometric complementarity.

Authors:  Carsten Berndt; Jens-Dirk Schwenn; Christopher Horst Lillig
Journal:  Chem Sci       Date:  2015-09-09       Impact factor: 9.825

10.  Substrate specificity of thioredoxins and glutaredoxins - towards a functional classification.

Authors:  Manuela Gellert; Md Faruq Hossain; Felix Jacob Ferdinand Berens; Lukas Willy Bruhn; Claudia Urbainsky; Volkmar Liebscher; Christopher Horst Lillig
Journal:  Heliyon       Date:  2019-12-17
  10 in total

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