| Literature DB >> 29526795 |
Kate E Keller1, Sanjoy K Bhattacharya2, Theresa Borrás3, Thomas M Brunner4, Sunee Chansangpetch5, Abbott F Clark6, W Michael Dismuke7, Yiqin Du8, Michael H Elliott9, C Ross Ethier10, Jennifer A Faralli11, Thomas F Freddo12, Rudolf Fuchshofer13, Michael Giovingo14, Haiyan Gong15, Pedro Gonzalez16, Alex Huang17, Murray A Johnstone18, Paul L Kaufman11, Mary J Kelley1, Paul A Knepper19, Casey C Kopczynski7, John G Kuchtey20, Rachel W Kuchtey20, Markus H Kuehn21, Raquel L Lieberman10, Shan C Lin5, Paloma Liton16, Yutao Liu22, Elke Lütjen-Drecoll23, Weiming Mao6, Marisse Masis-Solano5, Fiona McDonnell16, Colleen M McDowell6, Darryl R Overby24, Padmanabhan P Pattabiraman25, Vijay K Raghunathan26, P Vasanth Rao16, Douglas J Rhee25, Uttio Roy Chowdhury27, Paul Russell28, John R Samples29, Donald Schwartz30, Evan B Stubbs31, Ernst R Tamm13, James C Tan32, Carol B Toris25, Karen Y Torrejon33, Janice A Vranka1, Mary K Wirtz1, Thomas Yorio6, Jie Zhang17, Gulab S Zode6, Michael P Fautsch34, Donna M Peters35, Ted S Acott36, W Daniel Stamer37.
Abstract
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.Entities:
Keywords: Aqueous humor dynamics; Conventional outflow; Glaucoma; Intraocular pressure; Ocular hypertension
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Year: 2018 PMID： 29526795 PMCID： PMC6042513 DOI： 10.1016/j.exer.2018.03.001
Source DB: PubMed Journal: Exp Eye Res ISSN： 0014-4835 Impact factor: 3.770