Nami Yamashita1, Eriko Tokunaga2, Makoto Iimori3, Yuka Inoue4, Kimihiro Tanaka4, Hiroyuki Kitao3, Hiroshi Saeki4, Eiji Oki4, Yoshihiko Maehara4. 1. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. Electronic address: namanami@surg2.med.kyushu-u.ac.jp. 2. Department of Breast Oncology, National Hospital Organization, Kyushu Cancer Center, Fukuoka, Japan. 3. Department of Molecular Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 4. Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Abstract
BACKGROUND: E-cadherin and vimentin are regarded as major conventional canonical markers of the epithelial-mesenchymal transition. It is commonly assumed that E-cadherin is uniformly lost during the process of epithelial-mesenchymal transition. Breast tumor cells typically invade as a cohesive multicellular unit in a process called collective invasion. The aim of this study was to reveal the clinical importance of the expression pattern of E-cadherin and vimentin in breast cancer. METHODS: E-cadherin and vimentin protein expression was evaluated by immunohistochemistry in 176 invasive breast cancer samples. Among these, E-cadherin and vimentin expression were evaluated in the set of primary site and metastatic lymph nodes in 65 cases. In addition, E-cadherin and vimentin expression were analyzed by confocal laser scanning microscopy to see E-cadherin and vimentin localization in the breast cancer cells. RESULTS: Both at the primary site and metastatic lymph nodes, both E-cadherin- and vimentin-positive tumors had the worst disease-free and overall survival among all cases. In addition, E-cadherin and vimentin protein is colocalized within the same tumor cells in a human breast cancer specimen. CONCLUSION: Our present data suggest the existence of an aggressive subpopulation in the primary tumor nest of breast cancer.
BACKGROUND:E-cadherin and vimentin are regarded as major conventional canonical markers of the epithelial-mesenchymal transition. It is commonly assumed that E-cadherin is uniformly lost during the process of epithelial-mesenchymal transition. Breast tumor cells typically invade as a cohesive multicellular unit in a process called collective invasion. The aim of this study was to reveal the clinical importance of the expression pattern of E-cadherin and vimentin in breast cancer. METHODS:E-cadherin and vimentin protein expression was evaluated by immunohistochemistry in 176 invasive breast cancer samples. Among these, E-cadherin and vimentin expression were evaluated in the set of primary site and metastatic lymph nodes in 65 cases. In addition, E-cadherin and vimentin expression were analyzed by confocal laser scanning microscopy to see E-cadherin and vimentin localization in the breast cancer cells. RESULTS: Both at the primary site and metastatic lymph nodes, both E-cadherin- and vimentin-positive tumors had the worst disease-free and overall survival among all cases. In addition, E-cadherin and vimentin protein is colocalized within the same tumor cells in a humanbreast cancer specimen. CONCLUSION: Our present data suggest the existence of an aggressive subpopulation in the primary tumor nest of breast cancer.
Authors: Katarina Bartalska; Verena Hübschmann; Medina Korkut-Demirbaş; Ryan John A Cubero; Alessandro Venturino; Karl Rössler; Thomas Czech; Sandra Siegert Journal: iScience Date: 2022-06-11
Authors: Eike Burandt; Felix Lübbersmeyer; Natalia Gorbokon; Franziska Büscheck; Andreas M Luebke; Anne Menz; Martina Kluth; Claudia Hube-Magg; Andrea Hinsch; Doris Höflmayer; Sören Weidemann; Christoph Fraune; Katharina Möller; Frank Jacobsen; Patrick Lebok; Till Sebastian Clauditz; Guido Sauter; Ronald Simon; Ria Uhlig; Waldemar Wilczak; Stefan Steurer; Sarah Minner; Rainer Krech; David Dum; Till Krech; Andreas Holger Marx; Christian Bernreuther Journal: Biomark Res Date: 2021-06-05
Authors: Ilana Berger Fridman; James Kostas; Michal Gregus; Somak Ray; Matthew R Sullivan; Alexander R Ivanov; Smadar Cohen; Tania Konry Journal: Acta Biomater Date: 2021-06-18 Impact factor: 10.633