| Literature DB >> 29526620 |
Francesca Felice1, Anna Maria Piras2, Silvia Rocchiccioli3, Maria Chiara Barsotti1, Tatiana Santoni1, Angela Pucci4, Silvia Burchielli5, Federica Chiellini2, Nadia Ucciferri3, Roberto Solaro2, Angelina Altomare2, Antonella Cecchettini6, Rossella Di Stefano7.
Abstract
Endothelial progenitor cells (EPCs) contribute to ischemic tissue repair by paracrine secretion up-regulated by hypoxia. In this study we use novel nanoparticles (NPs) as carriers for a controlled release of EPC secretome (CM) to improve their angiogenic properties. The in vivo effect in ischemic hindlimb rat model was evaluated, comparing hypoxic EPC-CM-NPs with hypoxic EPC-CM alone. A proteomic characterization of hypoxic CM and the in vitro effect on endothelial cells (HUVECs) were also performed. Up to 647 protein, 17 of which with angiogenic properties, were upregulated by hypoxia. Moreover, hypoxic EPC-CM significantly promoted capillary-like structures on Matrigel. A significant increase of blood perfusion in ischemic limbs at 2 weeks with EPC-CM-loaded NPs as compared to both EPC-CM and control and a significant increase of capillary formation were observed. The use of EPC-CM-NPs significantly improved neoangiogenesis in vivo, underlining the advantages of controlled release in regenerative medicine.Entities:
Keywords: Angiogenesis; Conditioned medium; Endothelial progenitor cells; Hindlimb ischemia; Hypoxia; Nanoparticles
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Year: 2018 PMID： 29526620 DOI： 10.1016/j.ijpharm.2018.03.015
Source DB: PubMed Journal: Int J Pharm ISSN： 0378-5173 Impact factor: 5.875