Evaluation of the antimicrobial efficacy of neutral electrolyzed water on pork products and the formation of viable but nonculturable (VBNC) pathogens.

The goals of this study were to evaluate the antimicrobial efficacy of Neutral electrolyzed oxidizing (NEO) water on E. coli O157:H7, Salmonella Enteritidis and Yersinia enterocolitica in both pure culture and on inoculated pork chops and skin samples, and to investigate the formation of viable but nonculturable (VBNC) pathogens after treatments. Both the plate count method and flow cytometry were used to evaluate antimicrobial efficacy on pure cultures. Different concentrations of NEO water were prepared by diluting the original NEO water (100%) with sterilized deionized water. The antimicrobial efficacy increased as the concentrations of NEO water increased. The flow cytometry results showed that treating with diluted NEO water led to the formation of VBNC cells. No VBNC cells formed when treating pure cultures with 50% or 100% NEO water. Yersinia cultures were found to be more resistant to NEO treatments than Salmonella and E. coli O157:H7 cultures, with Yersinia cultures showing lower reductions and higher levels of VBNC cells after treatments. The antimicrobial efficacy of NEO water was significantly better on skin samples than on pork chops. The differences in protein content and structure between pork chops and skin samples serve as major factors impacting the NEO water's efficacy.