Literature DB >> 29525955

Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development.

Paolo Baesso1, Ricardo S Randall1, Giovanni Sena2.   

Abstract

Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.

Entities:  

Keywords:  Arabidopsis root; Control software; Live imaging; Microscopy; Morphodynamics; Tracking

Mesh：

Year:  2018        PMID： 29525955     DOI： 10.1007/978-1-4939-7747-5_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Live Plant Cell Tracking: Fiji plugin to analyze cell proliferation dynamics and understand morphogenesis.

Authors:  Paul Hernández-Herrera; Yamel Ugartechea-Chirino; Héctor H Torres-Martínez; Alejandro V Arzola; José Eduardo Chairez-Veloz; Berenice García-Ponce; María de la Paz Sánchez; Adriana Garay-Arroyo; Elena R Álvarez-Buylla; Joseph G Dubrovsky; Gabriel Corkidi
Journal:  Plant Physiol       Date:  2022-02-04       Impact factor: 8.340

2.  Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere.

Authors:  Yangminghao Liu; Daniel Patko; Ilonka Engelhardt; Timothy S George; Nicola R Stanley-Wall; Vincent Ladmiral; Bruno Ameduri; Tim J Daniell; Nicola Holden; Michael P MacDonald; Lionel X Dupuy
Journal:  Proc Natl Acad Sci U S A       Date:  2021-11-30       Impact factor: 11.205

3.  Imaging plant cells and organs with light-sheet and super-resolution microscopy.

Authors:  Miroslav Ovečka; Jiří Sojka; Michaela Tichá; George Komis; Jasim Basheer; Cintia Marchetti; Olga Šamajová; Lenka Kuběnová; Jozef Šamaj
Journal:  Plant Physiol       Date:  2022-02-04       Impact factor: 8.340
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.