Nancy J Lin1, Courtney Keeler2, Alison M Kraigsley3, Jing Ye4, Sheng Lin-Gibson5. 1. Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8543, Gaithersburg, MD 20878, USA. Electronic address: nancy.lin@nist.gov. 2. Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8543, Gaithersburg, MD 20878, USA. Electronic address: campbeco@up.edu. 3. Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8543, Gaithersburg, MD 20878, USA. Electronic address: kraigsleyam@niaid.nih.gov. 4. Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8543, Gaithersburg, MD 20878, USA. Electronic address: jye@wsgr.com. 5. Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Drive, MS 8543, Gaithersburg, MD 20878, USA.
Abstract
OBJECTIVE: Resin-based composites are known to elute leachables that include unincorporated starting materials. The objective of this work was to determine the effect of common dental monomers and initiators on Streptococcus mutans biofilm metabolic activity and biomass. METHODS: S. mutans biofilms were inoculated in the presence of bisphenol A glycerolate dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), camphorquinone (CQ), and ethyl 4-(dimethylamino)benzoate (4E) at 0.01μg/mL up to 500μg/mL, depending on the aqueous solubility of each chemical. Biofilms were evaluated at 4h and 24h for pH (n=3-8), biomass via crystal violet (n=12), metabolic activity via tetrazolium salt (n=12), and membrane permeability for selected concentrations via confocal microscopy (n=6). Parametric and non-parametric statistics were applied. RESULTS: 500μg/mL TEGDMA reduced 24h metabolic activity but not biomass, similar to prior results with leachables from undercured BisGMA-TEGDMA polymers. 50μg/mL BisGMA reduced biofilm biomass and activity, slightly delayed the pH drop, and decreased the number of cells with intact membranes. 100μg/mL CQ delayed the pH drop and metabolic activity at 4h but then significantly increased the 24h metabolic activity. 4E had no effect up to 10μg/mL. SIGNIFICANCE: Monomers and initiators that leach from resin composites affect oral bacterial biofilm growth in opposite ways. Leachables, which can be released for extended periods of time, have the potential to alter oral biofilm biomass and activity and should be considered in developing and evaluating new dental materials. Published by Elsevier Inc.
OBJECTIVE: Resin-based composites are known to elute leachables that include unincorporated starting materials. The objective of this work was to determine the effect of common dental monomers and initiators on Streptococcus mutans biofilm metabolic activity and biomass. METHODS:S. mutans biofilms were inoculated in the presence of bisphenol Aglycerolate dimethacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), camphorquinone (CQ), and ethyl 4-(dimethylamino)benzoate (4E) at 0.01μg/mL up to 500μg/mL, depending on the aqueous solubility of each chemical. Biofilms were evaluated at 4h and 24h for pH (n=3-8), biomass via crystal violet (n=12), metabolic activity via tetrazolium salt (n=12), and membrane permeability for selected concentrations via confocal microscopy (n=6). Parametric and non-parametric statistics were applied. RESULTS: 500μg/mL TEGDMA reduced 24h metabolic activity but not biomass, similar to prior results with leachables from undercured BisGMA-TEGDMA polymers. 50μg/mL BisGMA reduced biofilm biomass and activity, slightly delayed the pH drop, and decreased the number of cells with intact membranes. 100μg/mL CQ delayed the pH drop and metabolic activity at 4h but then significantly increased the 24h metabolic activity. 4E had no effect up to 10μg/mL. SIGNIFICANCE: Monomers and initiators that leach from resin composites affect oral bacterial biofilm growth in opposite ways. Leachables, which can be released for extended periods of time, have the potential to alter oral biofilm biomass and activity and should be considered in developing and evaluating new dental materials. Published by Elsevier Inc.
Authors: Ana P Fugolin; Andreia B de Paula; Adam Dobson; Vincent Huynh; Rafael Consani; Jack L Ferracane; Carmem S Pfeifer Journal: Dent Mater Date: 2020-05-08 Impact factor: 5.304
Authors: A P P Fugolin; Oscar Navarro; Matthew G Logan; Vincent Huynh; Cristiane M França; Jack L Ferracane; Carmem S Pfeifer Journal: Acta Biomater Date: 2020-08-24 Impact factor: 8.947