Yongming Zhou1, Jingyun Zhang2, Shukun Wang3, Wen Xu1, Weili Liang4, Meiying Yan2, Duochun Wang2, Baowei Diao2, Bo Pang2, Xin Lu2, Fenxia Fan2, Jie Li2, Jing Lou2, Li Zhang2, Ruibai Wang2, Xiaoying Cui2, Meng Zhao2, Rui Wu2, Hongyan Cai2, Xiaoli Du2, Zhigang Cui2, Wenpeng Gu1, Rusong Yang1, Biao Kan5. 1. Yunnan Center for Disease Control and Prevention, Yunnan 650022, China. 2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. 3. Yuxi Center for Disease Control and Prevention, Yunnan 653100, China. 4. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. 5. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China. Electronic address: kanbiao@icdc.cn.
Abstract
OBJECTIVES: To conduct a one-year pathogen surveillance of acute diarrheal disease based on outpatient clinics in township hospitals in rural Hongta District of Yunnan Province, China. METHODS: Fecal specimens of acute diarrhea cases and relevant epidemiological information were collected. Salmonella, Shigella, Vibrio, Aeromonas, Plesiomonas shigelloides and diarrheogenic Escherichia coli (DEC) were examined. RESULTS: Among the 797 stool specimens sampled, 198 samples (24.8%) were positive in pathogen isolation, and 223 strains were isolated. The order of isolation rates from high to low were DEC, Aeromonas, P. shigelloides, Salmonella, Shigella and Vibrio. The overall positive rate in middle school students and preschool children was relatively high; while the overall positive rate of less than 1-year-old infants and above 55 years olds was relatively low. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE). Some cases had the same or very close onset time, and the isolates had similar PFGE patterns, suggesting a possible outbreak once occurred but was not detected by the current infectious disease reporting system. CONCLUSIONS: Pathogen infection and transmission in rapidly urbanized rural areas is a serious issue. There is a great need for a more sensitive and accurate mode of monitoring, reporting and outbreak identification of diarrheal disease.
OBJECTIVES: To conduct a one-year pathogen surveillance of acute diarrheal disease based on outpatient clinics in township hospitals in rural Hongta District of Yunnan Province, China. METHODS: Fecal specimens of acute diarrhea cases and relevant epidemiological information were collected. Salmonella, Shigella, Vibrio, Aeromonas, Plesiomonas shigelloides and diarrheogenic Escherichia coli (DEC) were examined. RESULTS: Among the 797 stool specimens sampled, 198 samples (24.8%) were positive in pathogen isolation, and 223 strains were isolated. The order of isolation rates from high to low were DEC, Aeromonas, P. shigelloides, Salmonella, Shigella and Vibrio. The overall positive rate in middle school students and preschool children was relatively high; while the overall positive rate of less than 1-year-old infants and above 55 years olds was relatively low. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE). Some cases had the same or very close onset time, and the isolates had similar PFGE patterns, suggesting a possible outbreak once occurred but was not detected by the current infectious disease reporting system. CONCLUSIONS: Pathogen infection and transmission in rapidly urbanized rural areas is a serious issue. There is a great need for a more sensitive and accurate mode of monitoring, reporting and outbreak identification of diarrheal disease.
Authors: Yi Gao; Houyu Li; Bo Yang; Xiaocheng Wei; Chunxue Zhang; Yan Xu; Xiangqun Zheng Journal: Environ Sci Pollut Res Int Date: 2021-04-10 Impact factor: 4.223
Authors: Bingdong Song; Weirong Hu; Wanxia Hu; Rong Yang; Danlin Li; Chunyu Guo; Zhengmei Xia; Jie Hu; Fangbiao Tao; Jun Fang; Shichen Zhang Journal: Int J Environ Res Public Health Date: 2020-03-23 Impact factor: 3.390