Nurullah Bülbüller1, Barış Rafet Karakaş2, Hülya Tosun Yıldırım3, Muhittin Yaprak4, Veli Vural5, Sadıka Halide Akbaş6, Arzu Karaveli7, Cem Sezer8. 1. Full Professor, Department of General Surgery, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Conception and design of the study; acquisition, analysis and interpretation of data; manuscript preparation; critical revision. 2. Associate Professor, Department of General Surgery, Antalya Training and Research Hospital, Health Sciences University, Antalya, Turkey. Conception and design of the study; acquisition, analysis and interpretation of data; manuscript preparation; critical revision. 3. MD, Department of Pathology, Antalya Training and Research Hospital, Health Sciences University, Antalya, Turkey. Acquisition of data, manuscript preparation. 4. Assistant Professor, Department of General Surgery, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Acquisition, analysis and interpretation of data; manuscript preparation. 5. MD, Department of General Surgery, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Conception of the study, acquisition of data, manuscript preparation. 6. Full Professor, Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Acquisition, analysis and interpretation of data; manuscript preparation. 7. MD, Department of Anesthesiology and Reanimation, Antalya Training and Research Hospital, Health Sciences University, Antalya, Turkey. Conception of the study, acquisition of data, manuscript preparation. 8. Associate Professor, Department of Pathology, Antalya Training and Research Hospital, Health Sciences University, Antalya, Turkey. Conception and design of the study, analysis and interpretation of data, manuscript preparation, critical revision.
Abstract
PURPOSE: To evaluate the effect of a new cross-linked hyaluronan (NCHA) gel on healing of the staple line in an experimental sleeve gastrectomy. METHODS: Eighteen rats were randomly divided into three groups. The control group (n = 6) received no medication. In the saline group (n = 6) and NCHA gel group (n = 6), saline and NCHA gel were respectively administered onto the staple line and intraperitoneally into the abdominal cavity after the standard stapling procedure. RESULTS: The fibroblast activity and collagen deposition were significantly higher in the NCHA gel group than in the control group (p = 0.00, p = 0.017) and saline group (p = 0.004, p = 0.015). The tissue hydroxyproline protein level was significantly higher in the NCHA gel group than in the control group (p = 0.041). Adhesion formation was significantly lower in the NCHA gel group than in the control and saline groups (p = 0.015, p = 0.041). CONCLUSIONS: New cross-linked hyaluronan gel could be an effective approach to improve staple line wound healing and prevent potential leakage after sleeve gastrectomy. Moreover, NCHA gel helps to prevent adhesion formation without compromising healing of the staple line.
PURPOSE: To evaluate the effect of a new cross-linked hyaluronan (NCHA) gel on healing of the staple line in an experimental sleeve gastrectomy. METHODS: Eighteen rats were randomly divided into three groups. The control group (n = 6) received no medication. In the saline group (n = 6) and NCHA gel group (n = 6), saline and NCHA gel were respectively administered onto the staple line and intraperitoneally into the abdominal cavity after the standard stapling procedure. RESULTS: The fibroblast activity and collagen deposition were significantly higher in the NCHA gel group than in the control group (p = 0.00, p = 0.017) and saline group (p = 0.004, p = 0.015). The tissue hydroxyproline protein level was significantly higher in the NCHA gel group than in the control group (p = 0.041). Adhesion formation was significantly lower in the NCHA gel group than in the control and saline groups (p = 0.015, p = 0.041). CONCLUSIONS: New cross-linked hyaluronan gel could be an effective approach to improve staple line wound healing and prevent potential leakage after sleeve gastrectomy. Moreover, NCHA gel helps to prevent adhesion formation without compromising healing of the staple line.