Literature DB >> 29511439

Knockout of the placenta specific 8 gene radiosensitizes nasopharyngeal carcinoma cells by activating the PI3K/AKT/GSK3β pathway.

Rui Yang1, Ze-Zhang Tao1,2, Mao-Ling Huang1, Yong-Fa Zheng3, Meng-Yuan Dai1,4, You Zou1, Shi-Ming Chen1,2.   

Abstract

The present study investigated the radiosensitizing effect of placenta specific 8 (PLAC8) in nasopharyngeal carcinoma (NPC) cells. A PLAC8-knockout CNE2 cell line was constructed using the CRISPR/Cas9 system. The CCK-8 assay demonstrated knockout of PLAC8 significantly reduced cell proliferation and cell survival after irradiation compared to both control cells and non-irradiated PLAC8-knockout cells. The clonogenic assay showed knockout of PLAC8 enhanced the radiosensitivity of NPC cells. Flow cytometry revealed knockout of PLAC8 increased apoptosis and G2/M phase arrest after irradiation. Western blotting demonstrated knockout of PLAC8 was associated with increased levels of γHA2X, a higher BAX:BCL-2 ratio, and increased levels of phosphorylated-Akt and phosphorylated-GSK-3β. Overall, this study indicates PLAC8 contributes to radioresistance in NPC by inhibiting the PI3K/AKT/GSK3β pathway.

Entities:  

Keywords:  Apoptosis; PI3K/Akt/GSK3β pathway; knockout; nasopharyngeal carcinoma; placenta specific 8; radiosensitivity

Year:  2018        PMID： 29511439      PMCID： PMC5835810     

Source DB:  PubMed          Journal:  Am J Transl Res            Impact factor:   4.060


  30 in total

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Journal:  Curr Opin Oncol       Date:  2011-05       Impact factor: 3.645

2.  Onzin, a c-Myc-repressed target, promotes survival and transformation by modulating the Akt-Mdm2-p53 pathway.

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Authors:  Julie G Ledford; Martina Kovarova; Beverly H Koller
Journal:  J Immunol       Date:  2007-04-15       Impact factor: 5.422

4.  Dexmedetomidine attenuates propofol-induce neuroapoptosis partly via the activation of the PI3k/Akt/GSK3β pathway in the hippocampus of neonatal rats.

Authors:  Jing Lv; Yi Wei; Yanhua Chen; Xu Zhang; Zheng Gong; Yage Jiang; Qin Gong; Lifang Zhou; Hao Wang; Yubo Xie
Journal:  Environ Toxicol Pharmacol       Date:  2017-03-28       Impact factor: 4.860

5.  Glycogen synthase kinase 3beta induces apoptosis in cancer cells through increase of survivin nuclear localization.

Authors:  Jiansha Li; Mingyou Xing; Min Zhu; Xi Wang; Manxiang Wang; Sheng Zhou; Naping Li; Renliang Wu; Muxiang Zhou
Journal:  Cancer Lett       Date:  2008-08-12       Impact factor: 8.679

6.  Plac8 and Plac9, novel placental-enriched genes identified through microarray analysis.

Authors:  Carlos Galaviz-Hernandez; Carole Stagg; Gustaaf de Ridder; Tetsuya S Tanaka; Minoru S H Ko; David Schlessinger; Ramaiah Nagaraja
Journal:  Gene       Date:  2003-05-08       Impact factor: 3.688

7.  Apoptosis suppression by candidate oncogene PLAC8 is reversed in other cell types.

Authors:  Mirna Mourtada-Maarabouni; David Watson; Mamoona Munir; Farzin Farzaneh; Gwyn T Williams
Journal:  Curr Cancer Drug Targets       Date:  2013-01       Impact factor: 3.428

8.  Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer.

Authors:  Cunxi Li; Haiting Ma; Yang Wang; Zheng Cao; Ramona Graves-Deal; Anne E Powell; Alina Starchenko; Gregory D Ayers; Mary Kay Washington; Vidya Kamath; Keyur Desai; Michael J Gerdes; Lila Solnica-Krezel; Robert J Coffey
Journal:  J Clin Invest       Date:  2014-04-01       Impact factor: 14.808

9.  Plac8 is required for white adipocyte differentiation in vitro and cell number control in vivo.

Authors:  Maria Jimenez-Preitner; Xavier Berney; Bernard Thorens
Journal:  PLoS One       Date:  2012-11-14       Impact factor: 3.240

10.  Twist2 contributes to cisplatin-resistance of ovarian cancer through the AKT/GSK-3β signaling pathway.

Authors:  Tian Wang; Yan Li; Abidan Tuerhanjiang; Wenwen Wang; Zhangying Wu; Ming Yuan; Mayinuer Maitituoheti; Shixuan Wang
Journal:  Oncol Lett       Date:  2014-01-21       Impact factor: 2.967
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  4 in total

1.  PLAC8 gene knockout increases the radio-sensitivity of xenograft tumors in nude mice with nasopharyngeal carcinoma by promoting apoptosis.

Authors:  Li-Jun Shen; Cheng-Lin Qi; Rui Yang; Mao-Ling Huang; You Zou; Yang Jiang; Jian-Fei Sheng; Yong-Gang Kong; Qing-Quan Hua; Shi-Ming Chen
Journal:  Am J Transl Res       Date:  2021-06-15       Impact factor: 4.060

2.  Regulation of tamoxifen sensitivity by the PLAC8/MAPK pathway axis is antagonized by curcumin-induced protein stability change.

Authors:  Misha Mao; Dengdi Hu; Jingjing Yang; Yongxia Chen; Xun Zhang; Jianguo Shen; Rongyue Teng; Jichun Zhou; Linbo Wang
Journal:  J Mol Med (Berl)       Date:  2021-02-21       Impact factor: 4.599

3.  Plac8-mediated autophagy regulates nasopharyngeal carcinoma cell function via AKT/mTOR pathway.

Authors:  Mao-Ling Huang; Cheng-Lin Qi; You Zou; Rui Yang; Yang Jiang; Jian-Fei Sheng; Yong-Gang Kong; Ze-Zhang Tao; Shi-Ming Chen
Journal:  J Cell Mol Med       Date:  2020-05-29       Impact factor: 5.310

4.  Overexpression of Notch2 enhances radiosensitivity via inhibition of the AKT/mTOR signaling pathway in nasopharyngeal carcinoma.

Authors:  Li-Zhi Wu; Mao-Ling Huang; Cheng-Lin Qi; Li-Jun Shen; You Zou; Rui Yang; Jian-Fei Sheng; Shi-Ming Chen
Journal:  Bioengineered       Date:  2021-12       Impact factor: 3.269
  4 in total

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