Literature DB >> 29510884

Evaluation of a real-time PCR assay for detection of Mycoplasma genitalium and macrolide resistance-mediating mutations from clinical specimens.

Li Xiao1, Ken B Waites2, Hong Wang3, Barbara Van Der Pol4, Amy E Ratliff5, William M Geisler6.   

Abstract

Mycoplasma genitalium (MG) is a sexually transmitted pathogen for which there is no FDA-approved diagnostic test available in the United States. A modified real-time polymerase chain reaction assay for detecting MG and simultaneously identifying macrolide resistance mutations from clinical specimens was evaluated and proved to be sensitive and accurate for diagnostic purposes.
Copyright © 2018. Published by Elsevier Inc.

Entities:  

Keywords:  Macrolide; Mycoplasma genitalium; Real-time PCR; Resistance

Mesh：

Substances：

Year:  2018        PMID： 29510884      PMCID： PMC5970045          DOI： 10.1016/j.diagmicrobio.2018.02.002

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  12 in total

1.  Touchdown PCR for increased specificity and sensitivity in PCR amplification.

Authors:  Darren J Korbie; John S Mattick
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

2.  A 5' Nuclease Genotyping Assay for Identification of Macrolide-Resistant Mycoplasma genitalium in Clinical Specimens.

Authors:  Gitte Qvist Kristiansen; Jan Gorm Lisby; Kristian Schønning
Journal:  J Clin Microbiol       Date:  2016-04-06       Impact factor: 5.948

Review 3.  Molecular methods for the detection of Mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital Symposium on molecular pathology.

Authors:  Ken B Waites; Li Xiao; Vanya Paralanov; Rose M Viscardi; John I Glass
Journal:  J Mol Diagn       Date:  2012-07-20       Impact factor: 5.568

4.  Development of a quantitative real-time PCR assay for detection of Mycoplasma genitalium.

Authors:  Helle Friis Svenstrup; Jørgen Skov Jensen; Eva Björnelius; Peter Lidbrink; Svend Birkelund; Gunna Christiansen
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

5.  Identification of macrolide-resistant Mycoplasma genitalium using real-time PCR.

Authors:  C Wold; J Sorthe; U Hartgill; A O Olsen; A Moghaddam; N Reinton
Journal:  J Eur Acad Dermatol Venereol       Date:  2015-01-26       Impact factor: 6.166

6.  Direct detection of macrolide resistance in Mycoplasma genitalium isolates from clinical specimens from France by use of real-time PCR and melting curve analysis.

Authors:  Arabella Touati; Olivia Peuchant; Jorgen S Jensen; Cécile Bébéar; Sabine Pereyre
Journal:  J Clin Microbiol       Date:  2014-02-26       Impact factor: 5.948

7.  Transmission and selection of macrolide resistant Mycoplasma genitalium infections detected by rapid high resolution melt analysis.

Authors:  Jimmy Twin; Jorgen S Jensen; Catriona S Bradshaw; Suzanne M Garland; Christopher K Fairley; Lim Yi Min; Sepehr N Tabrizi
Journal:  PLoS One       Date:  2012-04-20       Impact factor: 3.240

8.  Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology.

Authors:  Sepehr N Tabrizi; Lit Y Tan; Samantha Walker; Jimmy Twin; Marin Poljak; Catriona S Bradshaw; Christopher K Fairley; Melanie Bissessor; Elisa Mokany; Alison V Todd; Suzanne M Garland
Journal:  PLoS One       Date:  2016-06-06       Impact factor: 3.240

9.  A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples.

Authors:  Marianne Gossé; Hilde Lysvand; Brita Pukstad; Svein Arne Nordbø
Journal:  J Clin Microbiol       Date:  2016-08-03       Impact factor: 5.948
View more
  4 in total

1.  Mycoplasma genitalium Infections With Macrolide and Fluoroquinolone Resistance-Associated Mutations in Heterosexual African American Couples in Alabama.

Authors:  Li Xiao; Ken B Waites; Barbara Van Der Pol; Kristal J Aaron; Edward W Hook; William M Geisler
Journal:  Sex Transm Dis       Date:  2019-01       Impact factor: 2.830

2.  Mycoplasma genitalium Coinfection in Women With Chlamydia trachomatis Infection.

Authors:  Sally A Harrison; Kristin M Olson; Amy E Ratliff; Li Xiao; Barbara Van Der Pol; Ken B Waites; William M Geisler
Journal:  Sex Transm Dis       Date:  2019-10       Impact factor: 2.830

3.  Mycoplasma genitalium Detection in Urogenital Specimens from Symptomatic and Asymptomatic Men and Women by Use of the cobas TV/MG Test.

Authors:  Barbara Van Der Pol; Ken B Waites; Li Xiao; Stephanie N Taylor; Arundhati Rao; Melinda Nye; Steven Chavoustie; Aaron Ermel; Clair Kaplan; David Eisenberg; Philip A Chan; Leandro Mena; Sixto Pacheco; Smitha Krishnamurthy; Ruchika Mohan; Rasa Bertuzis; Chris L McGowin; Rodney Arcenas; Elizabeth M Marlowe
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

4.  Mycoplasma genitalium Biofilms Contain Poly-GlcNAc and Contribute to Antibiotic Resistance.

Authors:  James M Daubenspeck; Arthur H Totten; Jason Needham; Monica Feng; Mitchell F Balish; T Prescott Atkinson; Kevin Dybvig
Journal:  Front Microbiol       Date:  2020-10-27       Impact factor: 5.640
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.