SCOPE: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. METHODS: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. 1 H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. RESULTS: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. CONCLUSIONS:Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.
RCT Entities:
SCOPE: The impact of dietary protein types on the gut microbiome is scarcely studied. The aim of the present study is therefore to examine the effects of lean-seafood and non-seafood proteins on the gut microbiome composition and activity and elucidate potential associations to cardiovascular disease (CVD) risk factors. METHODS: A crossover intervention study in which 20 healthy subjects consumed two diets that varied in protein source was conducted. 1 H NMR spectroscopy and 16S rDNA sequencing analyses were applied to characterize fecal metabolites and gut microbiota composition, respectively. RESULTS: A twofold increase in fecal trimethylamine excretion was observed after the lean-seafood diet period. Circulating TAG and the total to high-density lipoprotein (HDL) cholesterol ratio as well as circulating TMAO levels were each associated with specific gut bacteria. Following the non-seafood diet period, a decreased relative abundance of Clostridium cluster IV and a tendency toward an increased Firmicutes/Bacteroidetes ratio were found. CONCLUSIONS: Lean-seafood and non-seafood diets differentially modulate the gut microbiome composition and activity. Furthermore, the gut microbiota composition seems to affect circulating TMAO levels and CVD risk factors.
Authors: Eric B Rimm; Qi Sun; Jun Li; Yanping Li; Kerry L Ivey; Dong D Wang; Jeremy E Wilkinson; Adrian Franke; Kyu Ha Lee; Andrew Chan; Curtis Huttenhower; Frank B Hu Journal: Gut Date: 2021-04-29 Impact factor: 23.059
Authors: Zhendong Mei; Guo-Chong Chen; Zheng Wang; Mykhaylo Usyk; Bing Yu; Yoshiki Vazquez Baeza; Greg Humphrey; Rodolfo Salido Benitez; Jun Li; Jessica S Williams-Nguyen; Martha L Daviglus; Lifang Hou; Jianwen Cai; Yan Zheng; Rob Knight; Robert D Burk; Eric Boerwinkle; Robert C Kaplan; Qibin Qi Journal: Am J Clin Nutr Date: 2021-06-01 Impact factor: 7.045
Authors: J R Swann; M Rajilic-Stojanovic; A Salonen; O Sakwinska; C Gill; A Meynier; P Fança-Berthon; B Schelkle; N Segata; C Shortt; K Tuohy; O Hasselwander Journal: Eur J Nutr Date: 2020-04-03 Impact factor: 5.614