Anniina Rintala1,2, Iiris Riikonen1, Anne Toivonen3,4, Sami Pietilä5, Eveliina Munukka1, Juha-Pekka Pursiheimo6, Laura L Elo5, Pekka Arikoski7, Kristiina Luopajärvi8, Ursula Schwab9, Matti Uusitupa9, Seppo Heinonen10, Erkki Savilahti8, Erkki Eerola1,2, Jorma Ilonen1,11. 1. a Department of Medical Microbiology and Immunology , University of Turku , Turku , Finland. 2. b Department of Clinical Microbiology , Turku University Hospital , Turku , Finland. 3. c Department of Bacteriology and Immunology , University of Helsinki and Laboratory Services (HUSLAB), Division of Clinical Microbiology, Helsinki University Hospital , Helsinki , Finland. 4. d Department of Clinical Microbiology , Institute of Clinical Medicine, University of Eastern Finland , Kuopio , Finland. 5. e Turku Centre for Biotechnology , University of Turku and Åbo Akademi University , Turku , Finland. 6. f Turku Clinical Sequencing Laboratory , University of Turku , Turku , Finland. 7. g Department of Pediatrics , Kuopio University Hospital and University of Eastern Finland , Kuopio , Finland. 8. h Children's Hospital, Department of Pediatrics , Helsinki University Hospital and University of Helsinki , Helsinki , Finland. 9. i Institute of Public Health and Clinical Nutrition , University of Eastern Finland , Kuopio , Finland. 10. j Department of Obstetrics and Gynecology , Helsinki University Hospital and University of Helsinki , Helsinki , Finland. 11. k Immunogenetics Laboratory , Institute of Biomedicine, University of Turku , Turku , Finland.
Abstract
OBJECTIVES: Several studies have reported that the intestinal microbiota composition of celiac disease (CD) patients differs from healthy individuals. The possible role of gut microbiota in the pathogenesis of the disease is, however, not known. Here, we aimed to assess the possible differences in early fecal microbiota composition between children that later developed CD and healthy controls matched for age, sex and HLA risk genotype. MATERIALS AND METHODS: We used 16S rRNA gene sequencing to examine the fecal microbiota of 27 children with high genetic risk of developing CD. Nine of these children developed the disease by the age of 4 years. Stool samples were collected at the age of 9 and 12 months, before any of the children had developed CD. The fecal microbiota composition of children who later developed the disease was compared with the microbiota of the children who did not have CD or associated autoantibodies at the age of 4 years. Delivery mode, early nutrition, and use of antibiotics were taken into account in the analyses. RESULTS: No statistically significant differences in the fecal microbiota composition were found between children who later developed CD (n = 9) and the control children without disease or associated autoantibodies (n = 18). CONCLUSIONS: Based on our results, the fecal microbiota composition at the age of 9 and 12 months is not associated with the development of CD. Our results, however, do not exclude the possibility of duodenal microbiota changes or a later microbiota-related trigger for the disease.
OBJECTIVES: Several studies have reported that the intestinal microbiota composition of celiac disease (CD) patients differs from healthy individuals. The possible role of gut microbiota in the pathogenesis of the disease is, however, not known. Here, we aimed to assess the possible differences in early fecal microbiota composition between children that later developed CD and healthy controls matched for age, sex and HLA risk genotype. MATERIALS AND METHODS: We used 16S rRNA gene sequencing to examine the fecal microbiota of 27 children with high genetic risk of developing CD. Nine of these children developed the disease by the age of 4 years. Stool samples were collected at the age of 9 and 12 months, before any of the children had developed CD. The fecal microbiota composition of children who later developed the disease was compared with the microbiota of the children who did not have CD or associated autoantibodies at the age of 4 years. Delivery mode, early nutrition, and use of antibiotics were taken into account in the analyses. RESULTS: No statistically significant differences in the fecal microbiota composition were found between children who later developed CD (n = 9) and the control children without disease or associated autoantibodies (n = 18). CONCLUSIONS: Based on our results, the fecal microbiota composition at the age of 9 and 12 months is not associated with the development of CD. Our results, however, do not exclude the possibility of duodenal microbiota changes or a later microbiota-related trigger for the disease.
Authors: Anniina Keskitalo; Eveliina Munukka; Anna Aatsinki; Wisam Saleem; Noora Kartiosuo; Leo Lahti; Pentti Huovinen; Laura L Elo; Sami Pietilä; Suvi P Rovio; Harri Niinikoski; Jorma Viikari; Tapani Rönnemaa; Hanna Lagström; Antti Jula; Olli Raitakari; Katja Pahkala Journal: Nutrients Date: 2022-06-27 Impact factor: 6.706
Authors: Eveliina Munukka; Juha P Ahtiainen; Pere Puigbó; Sirpa Jalkanen; Katja Pahkala; Anniina Keskitalo; Urho M Kujala; Sami Pietilä; Maija Hollmén; Laura Elo; Pentti Huovinen; Giuseppe D'Auria; Satu Pekkala Journal: Front Microbiol Date: 2018-10-03 Impact factor: 5.640