Catarina Monteiro1,2, Patrícia I Marques1,2, Bruno Cavadas1,2, Isabel Damião3, Vasco Almeida3,4, Nuno Barros5, Alberto Barros1,5,6, Filipa Carvalho1,6, Sílvia Gomes1,2, Susana Seixas1,2. 1. Instituto de Investigação e Inovação em Saúde, Universidade do Porto (I3S), Porto, Portugal. 2. Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal. 3. Center of Infertility and Sterility Studies (CEIE), Porto, Portugal. 4. Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal. 5. Center for Reproductive Genetics Alberto Barros, Porto, Portugal. 6. Department of Genetics, Faculty of Medicine, University of Porto, Porto, Portugal.
Abstract
PROBLEM: Sexually transmitted diseases and other infections of male genitourinary tract are thought to negatively impact reproductive health, affecting semen quality. Despite a possible link between bacteria and infertility, few studies attempted to characterize seminal microbiota in healthy and diseased subjects. METHODS OF THE STUDY: A high-throughput sequencing of 16S ribosomal RNA gene was performed in a cohort of infertility-related cases (N = 89) and controls (N = 29) using a pooled sample approach. RESULTS: A global characterization of microbiota was obtained at low cost, without compromising the identification of bacterial taxa. This strategy allowed us to detect changes in the microbiota of infertility-related phenotypes, such as an increment of Proteobacteria in seminal hyperviscosity, and to separate this later group from oligoasthenoteratozoospermia based in bacterial (family/genus) abundances. CONCLUSION: We provide data for a likely contribution of bacteria into seminal hyperviscosity and oligoasthenoteratozoospermia, partially correlated with an increment of Neisseria, Klebsiella, and Pseudomonas pathogens and a reduction in Lactobacillus probiotic agent.
PROBLEM: Sexually transmitted diseases and other infections of male genitourinary tract are thought to negatively impact reproductive health, affecting semen quality. Despite a possible link between bacteria and infertility, few studies attempted to characterize seminal microbiota in healthy and diseased subjects. METHODS OF THE STUDY: A high-throughput sequencing of 16S ribosomal RNA gene was performed in a cohort of infertility-related cases (N = 89) and controls (N = 29) using a pooled sample approach. RESULTS: A global characterization of microbiota was obtained at low cost, without compromising the identification of bacterial taxa. This strategy allowed us to detect changes in the microbiota of infertility-related phenotypes, such as an increment of Proteobacteria in seminal hyperviscosity, and to separate this later group from oligoasthenoteratozoospermia based in bacterial (family/genus) abundances. CONCLUSION: We provide data for a likely contribution of bacteria into seminal hyperviscosity and oligoasthenoteratozoospermia, partially correlated with an increment of Neisseria, Klebsiella, and Pseudomonas pathogens and a reduction in Lactobacillus probiotic agent.
Authors: P I Marques; J C Gonçalves; C Monteiro; B Cavadas; L Nagirnaja; N Barros; A Barros; F Carvalho; A M Lopes; S Seixas Journal: Andrology Date: 2019-04-19 Impact factor: 3.842
Authors: Sergio Garcia-Segura; Javier Del Rey; Laia Closa; Iris Garcia-Martínez; Carlos Hobeich; Ana Belén Castel; Francisco Vidal; Jordi Benet; Jordi Ribas-Maynou; Maria Oliver-Bonet Journal: Front Cell Dev Biol Date: 2022-06-28
Authors: Jelena Štšepetova; Juliana Baranova; Jaak Simm; Ülle Parm; Tiiu Rööp; Sandra Sokmann; Paul Korrovits; Madis Jaagura; Karin Rosenstein; Andres Salumets; Reet Mändar Journal: Reprod Biol Endocrinol Date: 2020-01-16 Impact factor: 5.211