A Zullo1, E Simone2, M Grimaldi3, M Gagliardi4, L Zullo5, M R Matarazzo4, F P Mancini6. 1. Department of Sciences and Technologies, University of Sannio, Benevento, Italy; CEINGE Advanced Biotechnologies, Naples, Italy. Electronic address: albzullo@unisannio.it. 2. Department of Sciences and Technologies, University of Sannio, Benevento, Italy. 3. Department of Pediatric Oncology and Hematology, Charité University Hospital, Berlin, Germany. 4. Institute of Genetics and Biophysics 'Adriano Buzzati-Traverso', CNR, Naples, Italy. 5. Center for Synaptic Neuroscience and Technology (NSYN), IIT-Istituto Italiano di Tecnologia, Genova, Italy. 6. Department of Sciences and Technologies, University of Sannio, Benevento, Italy. Electronic address: mancini@unisannio.it.
Abstract
BACKGROUND AND AIM: Over the last decades advances in understanding the molecular bases of the close relationship between nutrition, metabolism, and diseases have been impressive. However, there are always novel frontiers coming up and epigenetics is one of these. Sirtuins, are pivotal factors in the control of metabolic pathways according to nutrient availability. In the present study we evaluated the effect of nutrient deprivation on expression, DNA methylation and chromatin status of the sirtuin genes. METHODS AND RESULTS: We performed these studies in mouse hepatoma cells, that were grown in standard medium, or in media containing low glucose concentration, or no glucose, or no amino acids. We applied quantitative real-time PCR to cDNA, methylation-enriched DNA and nuclease-treated DNA in order to evaluate gene expression, DNA methylation, and chromatin condensation, respectively. This study shows that the expression of sirtuin genes varies following nutrient deprivation. Moreover, we observed that changes of DNA methylation and chromatin condensation occur at the transcription start site of sirtuin genes following nutrient deprivation. CONCLUSIONS: Epigenetic mechanisms may have a role in the sirtuin response to nutrient deprivations in cultured hepatoma cells. Replicating these results in vivo to achieve a comprehensive understanding of the epigenetic control of sirtuin expression following nutrient deprivations might open up novel therapeutic possibilities to cure metabolic diseases and promote human health.
BACKGROUND AND AIM: Over the last decades advances in understanding the molecular bases of the close relationship between nutrition, metabolism, and diseases have been impressive. However, there are always novel frontiers coming up and epigenetics is one of these. Sirtuins, are pivotal factors in the control of metabolic pathways according to nutrient availability. In the present study we evaluated the effect of nutrient deprivation on expression, DNA methylation and chromatin status of the sirtuin genes. METHODS AND RESULTS: We performed these studies in mousehepatoma cells, that were grown in standard medium, or in media containing low glucose concentration, or no glucose, or no amino acids. We applied quantitative real-time PCR to cDNA, methylation-enriched DNA and nuclease-treated DNA in order to evaluate gene expression, DNA methylation, and chromatin condensation, respectively. This study shows that the expression of sirtuin genes varies following nutrient deprivation. Moreover, we observed that changes of DNA methylation and chromatin condensation occur at the transcription start site of sirtuin genes following nutrient deprivation. CONCLUSIONS: Epigenetic mechanisms may have a role in the sirtuin response to nutrient deprivations in cultured hepatoma cells. Replicating these results in vivo to achieve a comprehensive understanding of the epigenetic control of sirtuin expression following nutrient deprivations might open up novel therapeutic possibilities to cure metabolic diseases and promote human health.
Authors: Jaume Pérez-Sánchez; Paula Simó-Mirabet; Fernando Naya-Català; Juan Antonio Martos-Sitcha; Erick Perera; Azucena Bermejo-Nogales; Laura Benedito-Palos; Josep Alvar Calduch-Giner Journal: Front Endocrinol (Lausanne) Date: 2018-11-27 Impact factor: 5.555
Authors: Rochelle W Lai; Ryan Lu; Prakroothi S Danthi; Juan I Bravo; Alexandre Goumba; Nirmal Kumar Sampathkumar; Bérénice A Benayoun Journal: BMB Rep Date: 2019-01 Impact factor: 4.778