| Literature DB >> 29499499 |
Yujing Gao1, Gabrielle R Wilson1, Kiymet Bozaoglu1, Andrew G Elefanty2, Edouard G Stanley3, Mirella Dottori4, Paul J Lockhart5.
Abstract
Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers.Entities:
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Year: 2018 PMID： 29499499 DOI： 10.1016/j.scr.2018.02.015
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020