Literature DB >> 29497715

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range.

Y G Ermakova1, V V Pak, Y A Bogdanova, A A Kotlobay, I V Yampolsky, A G Shokhina, A S Panova, R A Marygin, D B Staroverov, D S Bilan, H Sies, V V Belousov.   

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

Entities:  

Year:  2018        PMID： 29497715     DOI： 10.1039/c7cc08740c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function.

Authors:  Valeriy V Pak; Daria Ezeriņa; Olga G Lyublinskaya; Brandán Pedre; Pyotr A Tyurin-Kuzmin; Natalie M Mishina; Marion Thauvin; David Young; Khadija Wahni; Santiago Agustín Martínez Gache; Alexandra D Demidovich; Yulia G Ermakova; Yulia D Maslova; Arina G Shokhina; Emrah Eroglu; Dmitry S Bilan; Ivan Bogeski; Thomas Michel; Sophie Vriz; Joris Messens; Vsevolod V Belousov
Journal:  Cell Metab       Date:  2020-03-03       Impact factor: 27.287

Review 2.  Genetically Encodable Fluorescent and Bioluminescent Biosensors Light Up Signaling Networks.

Authors:  Xin Zhou; Sohum Mehta; Jin Zhang
Journal:  Trends Biochem Sci       Date:  2020-07-10       Impact factor: 13.807

3.  TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy.

Authors:  Li Zhang; Felicia Dietsche; Bruno Seitaj; Liliana Rojas-Charry; Nadina Latchman; Dhanendra Tomar; Rob Ci Wüst; Alexander Nickel; Katrin Bm Frauenknecht; Benedikt Schoser; Sven Schumann; Michael J Schmeisser; Johannes Vom Berg; Thorsten Buch; Stefanie Finger; Philip Wenzel; Christoph Maack; John W Elrod; Jan B Parys; Geert Bultynck; Axel Methner
Journal:  Life Sci Alliance       Date:  2022-06-17

Review 4.  Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia.

Authors:  Alexander I Kostyuk; Aleksandra D Kokova; Oleg V Podgorny; Ilya V Kelmanson; Elena S Fetisova; Vsevolod V Belousov; Dmitry S Bilan
Journal:  Antioxidants (Basel)       Date:  2020-06-11

5.  ROS Live Cell Imaging During Neuronal Development.

Authors:  Aslihan Terzi; S M Sabbir Alam; Daniel M Suter
Journal:  J Vis Exp       Date:  2021-02-09       Impact factor: 1.355

Review 6.  Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.

Authors:  Ekaterina S Potekhina; Dina Y Bass; Ilya V Kelmanson; Elena S Fetisova; Alexander V Ivanenko; Vsevolod V Belousov; Dmitry S Bilan
Journal:  Int J Mol Sci       Date:  2020-12-25       Impact factor: 5.923

7.  Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives.

Authors:  Alexander I Kostyuk; Maria-Armineh Tossounian; Anastasiya S Panova; Marion Thauvin; Roman I Raevskii; Daria Ezeriņa; Khadija Wahni; Inge Van Molle; Anastasia D Sergeeva; Didier Vertommen; Andrey Yu Gorokhovatsky; Mikhail S Baranov; Sophie Vriz; Joris Messens; Dmitry S Bilan; Vsevolod V Belousov
Journal:  Nat Commun       Date:  2022-01-10       Impact factor: 14.919
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.