Dimitrios Tsoumas1, Sofia Nikou1, Efstathia Giannopoulou2, Spyridon Champeris Tsaniras3, Chaido Sirinian2, Ioannis Maroulis4, Stavros Taraviras3, Vassiliki Zolota5, Haralabos P Kalofonos2, Vasiliki Bravou6. 1. Department of Anatomy-Histology-Embryology, Medical School, University of Patras, Patras, Greece. 2. Clinical Oncology Laboratory, University of Patras Medical School, Patras, Greece. 3. Department of Physiology, Medical School, University of Patras, Patras, Greece. 4. Department of Surgery, University of Patras Medical School, Patras, Greece. 5. Department of Pathology, University of Patras Medical School, Patras, Greece. 6. Department of Anatomy-Histology-Embryology, Medical School, University of Patras, Patras, Greece vibra@upatras.gr.
Abstract
BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) and cancer stem cells (CSC) are critically implicated in cancer metastasis and chemoresistance. Herein, we investigated integrin-linked kinase (ILK)'s role in human colon cancer (CRC) progression and chemoresistance in relation to EMT and CSC markers. PATIENTS AND METHODS: Expression of ILK, EMT and CSC markers were evaluated by immunohistochemistry in 149 CRC samples. We also generated colon cancer cells resistant to 5-FU and oxaliplatin and studied the effect of ILK inhibition on drug response by MTT assay and on EMT and CSC markers' expression. RESULTS: ILK expression in human CRC correlates with EMT and CSC markers and is associated with metastasis and chemoresistance. ILK inhibition increases sensitivity of resistant cells to 5-FU and oxaliplatin and reduces the levels of EMT and CSC markers in 5-FU resistant cells. CONCLUSION: ILK overexpression in human CRC associates with EMT and CSC traits, contributing to tumor progression and chemoresistance. Copyright
BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) and cancer stem cells (CSC) are critically implicated in cancer metastasis and chemoresistance. Herein, we investigated integrin-linked kinase (ILK)'s role in humancolon cancer (CRC) progression and chemoresistance in relation to EMT and CSC markers. PATIENTS AND METHODS: Expression of ILK, EMT and CSC markers were evaluated by immunohistochemistry in 149 CRC samples. We also generated colon cancer cells resistant to 5-FU and oxaliplatin and studied the effect of ILK inhibition on drug response by MTT assay and on EMT and CSC markers' expression. RESULTS:ILK expression in human CRC correlates with EMT and CSC markers and is associated with metastasis and chemoresistance. ILK inhibition increases sensitivity of resistant cells to 5-FU and oxaliplatin and reduces the levels of EMT and CSC markers in 5-FU resistant cells. CONCLUSION:ILK overexpression in human CRC associates with EMT and CSC traits, contributing to tumor progression and chemoresistance. Copyright
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