| Literature DB >> 29492885 |
Armelle Marais1, Chantal Faure2, Bernard Bergey2, Thierry Candresse2.
Abstract
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.Keywords: Diagnostic; Double-stranded (ds) RNA; High-throughput sequencing; Metagenomics; NGS; Virome
Mesh:
Substances:
Year: 2018 PMID: 29492885 DOI: 10.1007/978-1-4939-7683-6_4
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745