Literature DB >> 29490239

Time to Stop Telling Biophysics Students that Light Is Primarily a Wave.

Philip C Nelson1.   

Abstract

Standard pedagogy introduces optics as though it were a consequence of Maxwell's equations and only grudgingly admits, usually in a rushed aside, that light has a particulate character that can somehow be reconciled with the wave picture. Recent revolutionary advances in optical imaging, however, make this approach more and more unhelpful: How are we to describe two-photon imaging, FRET, localization microscopy, and a host of related techniques to students who think of light primarily as a wave? I was surprised to find that everything I wanted my biophysics students to know about light, including image formation, x-ray diffraction, and even Bessel beams, could be expressed as well (or better) from the quantum viewpoint pioneered by Richard Feynman. Even my undergraduate students grasp this viewpoint as well as (or better than) the traditional one, and by mid-semester they are already well positioned to integrate the latest advances into their understanding. Moreover, I have found that this approach clarifies my own understanding of new techniques.
Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.

Mesh:

Year:  2018        PMID: 29490239      PMCID: PMC5984992          DOI: 10.1016/j.bpj.2017.12.036

Source DB:  PubMed          Journal:  Biophys J        ISSN: 0006-3495            Impact factor:   4.033


  6 in total

1.  Diffraction-free beams.

Authors: 
Journal:  Phys Rev Lett       Date:  1987-04-13       Impact factor: 9.161

2.  Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.

Authors:  Gleb Shtengel; James A Galbraith; Catherine G Galbraith; Jennifer Lippincott-Schwartz; Jennifer M Gillette; Suliana Manley; Rachid Sougrat; Clare M Waterman; Pakorn Kanchanawong; Michael W Davidson; Richard D Fetter; Harald F Hess
Journal:  Proc Natl Acad Sci U S A       Date:  2009-02-06       Impact factor: 11.205

3.  Imaging cellular ultrastructure by PALM, iPALM, and correlative iPALM-EM.

Authors:  Gleb Shtengel; Yilin Wang; Zhen Zhang; Wah Ing Goh; Harald F Hess; Pakorn Kanchanawong
Journal:  Methods Cell Biol       Date:  2014       Impact factor: 1.441

4.  3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy.

Authors:  Liang Gao; Lin Shao; Bi-Chang Chen; Eric Betzig
Journal:  Nat Protoc       Date:  2014-04-10       Impact factor: 13.491

5.  Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions.

Authors:  Lindsay B Case; Michelle A Baird; Gleb Shtengel; Sharon L Campbell; Harald F Hess; Michael W Davidson; Clare M Waterman
Journal:  Nat Cell Biol       Date:  2015-06-08       Impact factor: 28.824

6.  High-density three-dimensional localization microscopy across large volumes.

Authors:  Wesley R Legant; Lin Shao; Jonathan B Grimm; Timothy A Brown; Daniel E Milkie; Brian B Avants; Luke D Lavis; Eric Betzig
Journal:  Nat Methods       Date:  2016-03-07       Impact factor: 28.547

  6 in total

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