Dayanne Terra Tenório Nonato1, Silvânia Maria Mendes Vasconcelos2, Mário Rogério Lima Mota3, Paulo Goberlânio de Barros Silva4, Arcelina Pacheco Cunha5, Nágila Maria Pontes Silva Ricardo6, Maria Gonçalves Pereira7, Ana Maria Sampaio Assreuy8, Edna Maria Camelo Chaves9. 1. Superior Institute of Biomedical Sciences, State University of Ceara, Av. Dr. Silas Munguba, 1700, Itaperi, 60714-903, Fortaleza, CE, Brazil. Electronic address: dayanne.nonato@aluno.uece.br. 2. Departament of Physiology and Pharmacology, Federal University of Ceara, Rua Coronel Nunes Valente, 1127, Rodolfo Teófilo, 607430-970, Fortaleza, CE, Brazil. Electronic address: silvania@pesquisador.cnpq.br. 3. Department of Oral Pathology and Clinical Stomatology of Federal University of Ceara, Rua Coronel Nunes Valente, 1127, Rodolfo Teófilo, 607430-970, Fortaleza, CE, Brazil. Electronic address: mariolmota@ufc.br. 4. Department of Oral Pathology and Clinical Stomatology of Federal University of Ceara, Rua Coronel Nunes Valente, 1127, Rodolfo Teófilo, 607430-970, Fortaleza, CE, Brazil. Electronic address: coordodontopesquisa01@unichristus.edu.br. 5. Department of Organic and Inorganic Chemistry, Federal University of Ceara, Rua Humberto Monte, S/N, Campus de PICI, 60440554, Fortaleza, CE, Brazil. Electronic address: arcelinapacheco@yahoo.com.br. 6. Department of Organic and Inorganic Chemistry, Federal University of Ceara, Rua Humberto Monte, S/N, Campus de PICI, 60440554, Fortaleza, CE, Brazil. Electronic address: naricard@ufc.br. 7. Superior Institute of Biomedical Sciences, State University of Ceara, Av. Dr. Silas Munguba, 1700, Itaperi, 60714-903, Fortaleza, CE, Brazil; Faculty of Education Science and Letters of the Hinterland, Rua José de Queiroz Pessoa, 2554 - Planalto Universitário, 63.900-000, Quixadá, CE, Brazil. Electronic address: maria.pereira@uece.br. 8. Superior Institute of Biomedical Sciences, State University of Ceara, Av. Dr. Silas Munguba, 1700, Itaperi, 60714-903, Fortaleza, CE, Brazil. Electronic address: ana.assereuy@uece.br. 9. Superior Institute of Biomedical Sciences, State University of Ceara, Av. Dr. Silas Munguba, 1700, Itaperi, 60714-903, Fortaleza, CE, Brazil. Electronic address: edna.chaves@uece.br.
Abstract
BACKGROUND: This study aimed to chemically characterize a polysaccharide-rich extract (PRE) obtained from Genipa americana leaves and evaluate its neuroprotective effect in the brain morphology and oxidative markers using mice behavioral models. METHODS: Dry powder (5 g) of G. americana leaves were submitted to depigmentation in methanol. PRE was obtained by extraction in NaOH and precipitation with absolute ethanol and characterized by infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H and 13C NMR). Swiss mice (25-35 g) received saline (0.9% NaCl) or PRE (1-27 mg/kg) by intraperitoneal (i.p.) route, 30 min before evaluation in behavioral models (open field, elevated plus maze, sleeping time, tail suspension, forced swimming, seizures induced by pentylenetetrazole-PTZ). Animal's brain were dissected and analyzed for histological alterations and oxidative stress. RESULTS: FTIR spectrum showed bands around 3417 cm-1 and 2928 cm-1, relative to the vibrational stretching of OH and CH, respectively. 1H NMR spectrum revealed signals at δ 3.85 (methoxyl groups) and δ 2.4 (acetyl) ppm. 13C NMR spectrum revealed signals at δ 108.0 and δ 61.5 ppm, corresponding to C1 and C5 of α-L-arabinofuranosyl residues. PRE presented central inhibitory effect, increasing the latency for PTZ-induced seizures by 63% (9 mg/kg) and 55% (27 mg/kg), and the latency to death by 73% (9 mg/kg) and 72% (27 mg/kg). Both effects were reversed by the association with flumazenil. CONCLUSIONS: PRE, containing a heteropolysaccharide, presents antioxidant and anticonvulsant effect in the model of PTZ-induced seizures via gamma-aminobutyric acid (GABA), decreasing the number of hippocampal black neurons.
BACKGROUND: This study aimed to chemically characterize a polysaccharide-rich extract (PRE) obtained from Genipa americana leaves and evaluate its neuroprotective effect in the brain morphology and oxidative markers using mice behavioral models. METHODS: Dry powder (5 g) of G. americana leaves were submitted to depigmentation in methanol. PRE was obtained by extraction in NaOH and precipitation with absolute ethanol and characterized by infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H and 13C NMR). Swiss mice (25-35 g) received saline (0.9% NaCl) or PRE (1-27 mg/kg) by intraperitoneal (i.p.) route, 30 min before evaluation in behavioral models (open field, elevated plus maze, sleeping time, tail suspension, forced swimming, seizures induced by pentylenetetrazole-PTZ). Animal's brain were dissected and analyzed for histological alterations and oxidative stress. RESULTS: FTIR spectrum showed bands around 3417 cm-1 and 2928 cm-1, relative to the vibrational stretching of OH and CH, respectively. 1H NMR spectrum revealed signals at δ 3.85 (methoxyl groups) and δ 2.4 (acetyl) ppm. 13C NMR spectrum revealed signals at δ 108.0 and δ 61.5 ppm, corresponding to C1 and C5 of α-L-arabinofuranosyl residues. PRE presented central inhibitory effect, increasing the latency for PTZ-induced seizures by 63% (9 mg/kg) and 55% (27 mg/kg), and the latency to death by 73% (9 mg/kg) and 72% (27 mg/kg). Both effects were reversed by the association with flumazenil. CONCLUSIONS: PRE, containing a heteropolysaccharide, presents antioxidant and anticonvulsant effect in the model of PTZ-induced seizures via gamma-aminobutyric acid (GABA), decreasing the number of hippocampal black neurons.
Authors: Mário Rogério Lima Mota; José Ronildo Lins do Carmo Filho; Timna Varela Martins; Devany Quintela Soares; Mariana Pereira de Sousa; Paulo Goberlânio de Barros Silva; Ana Paula Negreiros Nunes Alves; Maria Gonçalves Pereira; Ana Maria Sampaio Assreuy Journal: Inflammopharmacology Date: 2022-08-03 Impact factor: 5.093
Authors: Aline T de Carvalho; Marina M Paes; Mila S Cunha; Gustavo C Brandão; Ana M Mapeli; Vanessa C Rescia; Silvia A Oesterreich; Gustavo R Villas-Boas Journal: Molecules Date: 2020-08-26 Impact factor: 4.411