Literature DB >> 2948593

Identification of two macrophage populations by flow cytometry monitoring of oxidative burst and phagocytic functions.

M Lepoivre, A C Roche, J P Tenu, J F Petit, D Nolibe, M Monsigny.   

Abstract

Two populations of phagocytic cells from trehalose dimycolate-elicited mouse peritoneal cells are demonstrated by flow cytofluorometry, using two fluorescent probes excited at the same wavelength (488 nm). Liposomes containing diethylenetriaminepentaacetate daunorubicin conjugate (maximum emission wavelength: 590 nm) allow the discrimination of phagocytes and non-phagocytic cells. Among the phagocytes, an activated population is revealed by a cell-associated fluorescence of the oxidation product of dichlorofluorescein diacetate (maximum emission wavelength: 520 nm).

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Year:  1986        PMID: 2948593     DOI: 10.1111/j.1768-322x.1986.tb00470.x

Source DB:  PubMed          Journal:  Biol Cell        ISSN: 0248-4900            Impact factor:   4.458


  3 in total

1.  Interaction of bovine respiratory syncytial virus with bovine alveolar macrophages in vivo: effects of virus infection upon selected cell functions.

Authors:  T W Olchowy; T R Ames; T W Molitor
Journal:  Can J Vet Res       Date:  1994-01       Impact factor: 1.310

2.  Dietary phenolic acids act as effective antioxidants in membrane models and in cultured cells, exhibiting proapoptotic effects in leukaemia cells.

Authors:  Laura Zambonin; Cristiana Caliceti; Francesco Vieceli Dalla Sega; Diana Fiorentini; Silvana Hrelia; Laura Landi; Cecilia Prata
Journal:  Oxid Med Cell Longev       Date:  2012-06-26       Impact factor: 6.543

3.  Fluoromicroscopic studies of bleomycin-induced intracellular oxidation in alveolar macrophages and its inhibition by taurine.

Authors:  M Bhat; Y Rojanasakul; S L Weber; J Y Ma; V Castranova; D E Banks; J K Ma
Journal:  Environ Health Perspect       Date:  1994-12       Impact factor: 9.031

  3 in total

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