Amina Khadjavi1, Ilaria Stura2, Mauro Prato3, Valerio Giacomo Minero4, Alice Panariti5, Ilaria Rivolta5, Giulia Rossana Gulino6, Federica Bessone7, Giuliana Giribaldi6, Elena Quaglino4, Roberta Cavalli7, Federica Cavallo4, Caterina Guiot1. 1. Dipartimento di Neuroscienze, Università di Torino, Corso Raffaello 30, 10125, Torino, Italy. 2. Dipartimento di Scienze della Sanità Pubblica e Pediatriche, Università di Torino, Via Santena 5 bis, Torino, 10126, Italy. ilaria.stura@unito.it. 3. Dipartimento di Scienze della Sanità Pubblica e Pediatriche, Università di Torino, Via Santena 5 bis, Torino, 10126, Italy. 4. Dipartimento di Biotecnologie Molecolari e Scienze per la Salute, Università di Torino, Torino, Italy. 5. Dipartimento di Medicina Sperimentale, Università Milano Bicocca, Monza, Italy. 6. Dipartimento di Oncologia, Università di Torino, Torino, Italy. 7. Dipartimento di Scienze e Tecnologia del Farmaco, Università di Torino, Torino, Italy.
Abstract
PURPOSE: Chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLN) are a new class of nanodevices to effectively deliver anti-cancer drugs to tumoral cells. This study investigated their antitumoral effects 'per se', using a mathematical model validated on experimental data. METHODS: OLN were prepared and characterized either in vitro or in vivo. TUBO cells, established from a lobular carcinoma of a BALB-neuT mouse, were investigated following 48 h of incubation in the absence/presence of different concentrations of OLN. OLN internalization, cell viability, necrosis, apoptosis, cell cycle and reactive oxygen species (ROS) production were checked as described in the Method section. In vivo tumor growth was evaluated after subcutaneous transplant in BALB/c mice of TUBO cells either without treatment or after 24 h incubation with 10% v/v OLN. RESULTS: OLN showed sizes of about 350 nm and a positive surface charge (45 mV). Dose-dependent TUBO cell death through ROS-triggered apoptosis following OLN internalization was detected. A mathematical model predicting the effects of OLN uptake was validated on both in vitro and in vivo results. CONCLUSIONS: Due to their intrinsic toxicity OLN might be considered an adjuvant tool suitable to deliver their therapeutic cargo intracellularly and may be proposed as promising combined delivery system.
PURPOSE:Chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLN) are a new class of nanodevices to effectively deliver anti-cancer drugs to tumoral cells. This study investigated their antitumoral effects 'per se', using a mathematical model validated on experimental data. METHODS:OLN were prepared and characterized either in vitro or in vivo. TUBO cells, established from a lobular carcinoma of a BALB-neuT mouse, were investigated following 48 h of incubation in the absence/presence of different concentrations of OLN. OLN internalization, cell viability, necrosis, apoptosis, cell cycle and reactive oxygen species (ROS) production were checked as described in the Method section. In vivo tumor growth was evaluated after subcutaneous transplant in BALB/c mice of TUBO cells either without treatment or after 24 h incubation with 10% v/v OLN. RESULTS:OLN showed sizes of about 350 nm and a positive surface charge (45 mV). Dose-dependent TUBO cell death through ROS-triggered apoptosis following OLN internalization was detected. A mathematical model predicting the effects of OLN uptake was validated on both in vitro and in vivo results. CONCLUSIONS: Due to their intrinsic toxicityOLN might be considered an adjuvant tool suitable to deliver their therapeutic cargo intracellularly and may be proposed as promising combined delivery system.
Entities:
Keywords:
antitumor nanodevice; breast cancer; chitosan nanodroplet; nanocarrier; oxygen
Authors: Ludwig K Limbach; Peter Wick; Pius Manser; Robert N Grass; Arie Bruinink; Wendelin J Stark Journal: Environ Sci Technol Date: 2007-06-01 Impact factor: 9.028