Maria D'Apolito1, Angelo Campanozzi1, Ida Giardino2, Massimo Pettoello-Mantovani1,3,4. 1. Pediatric Research Center, Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy. 2. Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy. 3. University Pediatric Unit, Department of Pediatrics, Scientific Institute "Casa Sollievo della Sofferenza", University of Foggia, Italy. 4. European Paediatric Association/Union of National Paediatric Societies and Associations (EPA/UNEPSA), Berlin, Germany.
Abstract
AIM: The aim of the study was to investigate the level of cytokines in cultures of cow's milk protein- stimulated peripheral blood mononuclear cells of patients with cow's milk protein allergy. MATERIAL AND METHODS: Eleven children with cow's milk protein allergy and 11 non-allergic controls were studied. Their peripheral blood mononuclear cells were cultured alone and in the presence of cow's milk α-lactalbumin; β-lactoglobulin; αS 1, αS 2, β, and κ-casein fraction mixtures; and a cow's protein mixture from whole milk. Production of cytokines, tumor necrosis factor-α, interleukin-10, and interleukin-12 were determined in culture supernatants. RESULTS: In cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children with cow's milk protein allergy, tumor necrosis factor-α, interleukin-10, and interleukin-12 production was significantly higher than in non-allergic controls (p<0.05). No difference in cytokine production was found between cultures obtained from unstimulated peripheral blood mononuclear cell cultures of both cow's milk protein allergy and non-allergic controls. CONCLUSIONS: The findings of this preliminary study align with data from the literature suggesting that the investigation of tumor necrosis factor-α, interleukin-10, and interleukin-12 in cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children may be taken in further consideration to explore whether they might have a predictive role for cow's milk protein allergy. Further studies are therefore needed to extensively investigate this issue.
AIM: The aim of the study was to investigate the level of cytokines in cultures of cow's milk protein- stimulated peripheral blood mononuclear cells of patients with cow's milk protein allergy. MATERIAL AND METHODS: Eleven children with cow's milk protein allergy and 11 non-allergic controls were studied. Their peripheral blood mononuclear cells were cultured alone and in the presence of cow's milk α-lactalbumin; β-lactoglobulin; αS 1, αS 2, β, and κ-casein fraction mixtures; and a cow's protein mixture from whole milk. Production of cytokines, tumor necrosis factor-α, interleukin-10, and interleukin-12 were determined in culture supernatants. RESULTS: In cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children with cow's milk protein allergy, tumor necrosis factor-α, interleukin-10, and interleukin-12 production was significantly higher than in non-allergic controls (p<0.05). No difference in cytokine production was found between cultures obtained from unstimulated peripheral blood mononuclear cell cultures of both cow's milk protein allergy and non-allergic controls. CONCLUSIONS: The findings of this preliminary study align with data from the literature suggesting that the investigation of tumor necrosis factor-α, interleukin-10, and interleukin-12 in cow's milk protein-stimulated peripheral blood mononuclear cell cultures of children may be taken in further consideration to explore whether they might have a predictive role for cow's milk protein allergy. Further studies are therefore needed to extensively investigate this issue.
Authors: Ruben Dario Motrich; Claudio Gottero; Carlos Rezzonico; Carlos Rezzonico; Clelia Maria Riera; Virginia Rivero Journal: Clin Immunol Date: 2003-11 Impact factor: 3.969
Authors: Machteld M Tiemessen; Adrie G Van Ieperen-Van Dijk; Carla A f m Bruijnzeel-Koomen; Johan Garssen; Edward F Knol; Els Van Hoffen Journal: J Allergy Clin Immunol Date: 2004-05 Impact factor: 10.793