I Alajbeg1,2, I Alić3,4, A Andabak-Rogulj1, V Brailo1,2, D Mitrečić5,6. 1. Department of Oral Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia. 2. Department of Dentistry, University Hospital Centre Zagreb, Zagreb, Croatia. 3. Department of Anatomy, Histology and Embryology, University of Zagreb Faculty of Veterinary Medicine, Zagreb, Croatia. 4. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore. 5. Department of Histology and Embryology, University of Zagreb School of Medicine, Zagreb, Croatia. 6. Laboratory for Stem Cells, Croatian Institute for Brain Research, Zagreb, Croatia.
Abstract
OBJECTIVE: To observe simultaneous differentiation and analyse possible interactions between co-cultured human oral mucosal stem cells (hOMSC) and mouse neural stem cells (mNSC). MATERIALS AND METHODS: hOMSC and mNSC were co-cultured in mouse and in human medium, and their immunocytochemical characterization to detect survival rate and differentiation pattern was performed. Co-cultures in different media were compared to hOMSC in human medium and mNSC in mouse medium as controls. RESULTS: Co-culture of hOMSC and mNSC in medium for human cells led to normal differentiation pattern of human cells, while mNSC were directed towards astrocytes. When the same cells were cultivated in the mouse medium, both cell types succeeded to form neurons, although mNSC showed a tendency to overgrow hOMSC. hOMSC alone in the human-specific medium differentiated towards ectodermal (Oct4, Map2) and mesodermal (Osterix) cell populations. mNSC in the mouse-specific medium differentiated towards Map2-, β3-tubulin- and NeuN-positive neurons. CONCLUSIONS: hOMSC and mNSC can form co-cultures. Different media considerably affected the differentiation pattern of co-cultures, whereas one cell population itself modestly influenced differentiation of the other cell type. The in vitro differentiation pattern of hOMSC in the mouse neural tissue environment suggested that hOMSC could be beneficial in the brain tissue affected by ischaemia.
OBJECTIVE: To observe simultaneous differentiation and analyse possible interactions between co-cultured human oral mucosal stem cells (hOMSC) and mouse neural stem cells (mNSC). MATERIALS AND METHODS: hOMSC and mNSC were co-cultured in mouse and in human medium, and their immunocytochemical characterization to detect survival rate and differentiation pattern was performed. Co-cultures in different media were compared to hOMSC in human medium and mNSC in mouse medium as controls. RESULTS: Co-culture of hOMSC and mNSC in medium for human cells led to normal differentiation pattern of human cells, while mNSC were directed towards astrocytes. When the same cells were cultivated in the mouse medium, both cell types succeeded to form neurons, although mNSC showed a tendency to overgrow hOMSC. hOMSC alone in the human-specific medium differentiated towards ectodermal (Oct4, Map2) and mesodermal (Osterix) cell populations. mNSC in the mouse-specific medium differentiated towards Map2-, β3-tubulin- and NeuN-positive neurons. CONCLUSIONS: hOMSC and mNSC can form co-cultures. Different media considerably affected the differentiation pattern of co-cultures, whereas one cell population itself modestly influenced differentiation of the other cell type. The in vitro differentiation pattern of hOMSC in the mouse neural tissue environment suggested that hOMSC could be beneficial in the brain tissue affected by ischaemia.
Authors: Marc Ruoß; Vanessa Kieber; Silas Rebholz; Caren Linnemann; Helen Rinderknecht; Victor Häussling; Marina Häcker; Leon H H Olde Damink; Sabrina Ehnert; Andreas K Nussler Journal: Methods Protoc Date: 2019-12-23
Authors: Dinko Mitrečić; Valentina Hribljan; Denis Jagečić; Jasmina Isaković; Federica Lamberto; Alex Horánszky; Melinda Zana; Gabor Foldes; Barbara Zavan; Augustas Pivoriūnas; Salvador Martinez; Letizia Mazzini; Lidija Radenovic; Jelena Milasin; Juan Carlos Chachques; Leonora Buzanska; Min Suk Song; András Dinnyés Journal: Int J Mol Sci Date: 2022-01-13 Impact factor: 6.208