| Literature DB >> 29480490 |
Aida Martinez-Sanchez1, Marie-Sophie Nguyen-Tu2, Isabelle Leclerc2, Guy A Rutter3.
Abstract
The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.Entities:
Keywords: AMPK activity; Insulin secretion; Islet isolation; Mouse and human islets; Pharmacological AMPK activators
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Year: 2018 PMID： 29480490 DOI： 10.1007/978-1-4939-7598-3_26
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745