| Literature DB >> 29480473 |
Pascual Sanz1, Rosa Viana2, Maria Adelaida Garcia-Gimeno3.
Abstract
Mammalian AMP-activated protein kinase (AMPK) is a Ser/Thr protein kinase that acts as a crucial energy sensor in the cell. Since AMPK plays a key role in a multitude of different pathways in the cell, major efforts have been concentrated to elucidate its signaling network, mainly by the identification of AMPK downstream targets. In this chapter we describe a yeast two-hybrid method for the direct evaluation of the interaction between an AMPK subunit and putative substrates.Entities:
Keywords: AMPK; Bait plasmid; Prey plasmid; Yeast two-hybrid; β-Galactosidase
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Year: 2018 PMID: 29480473 PMCID: PMC5895849 DOI: 10.1007/978-1-4939-7598-3_9
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745