Masoumeh Mehdipour1, Minoo Shahidi2, Soheila Manifar3, Soudeh Jafari4, Fatemeh Mashhadi Abbas5, Mahmood Barati6, Hamed Mortazavi1, Mohammad Shirkhoda7, Amir Farzanegan8, Zahra Elmi Rankohi9. 1. Department of Oral Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Hematology and blood banking Department, Faculty of Allied Medicine & Cellular & Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran. 3. Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Oral Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. SoudehJafari@yahoo.com. 5. Department of Oral Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 6. Department of Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. 7. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. 8. Research Center for Prevention of Oral and Dental Diseases, Baghiyatallah, University of Medical Sciences, Tehran, Iran. 9. Department of Oral and Maxillofacial Medicine, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
Abstract
BACKGROUND: Oral lichen planus (OLP), a relatively common chronic inflammatory disease of the oral mucosa, is considered to be a premalignant disorder of the oral cavity. Previously, several biomarkers have been tested for their diagnostic potential. Here, we aimed to investigate the diagnostic potential of four miRNAs, miR-21, -125a, -31 and -200a, known to be involved in oral squamous cell carcinoma (OSCC) development, in the saliva of OLP patients as also their putative relation to OSCC development in these patients. MATERIALS AND METHODS: Saliva samples from 30 patients with OLP were collected, 15 of whom were diagnosed with dysplasia upon histopathologic examination. In addition, 15 saliva samples from patients with OSCC and 15 saliva samples from healthy donors were collected. After RNA extraction, the respective miRNA levels were assessed by quantitative RT-PCR. RESULTS: We found that the miR-21 levels were significantly increased in saliva samples derived from patients with OLP, dysplastic OLP and OSCC, compared to those from healthy controls (p = 0.012, p = 0.0017 and p < 0.0001, respectively). Conversely, significant decreases in miR-125a levels were found in the OLP, dysplastic OLP and OSCC samples, compared to those from healthy controls (p < 0.0014, p < 0.0001 and p < 0.0001, respectively). In addition, significant increases in miR-31 levels were found in samples derived from dysplastic OLP and OSCC patients, but not in those from nondysplastic OLP patients, compared to those in healthy controls (p = 0.01 and p = 0.004, respectively). Finally, we found that the miR-200a levels were significantly decreased only in samples derived from OSCC patients (p < 0.0001). CONCLUSIONS: From our data we conclude that increased miR-21 levels in conjunction with decreased miR-125a levels in saliva of OLP patients may be indicative for a poor prognosis. Conversely, we conclude that lack of significant alterations in miR-31 and miR-200a levels in saliva of OLP patients may be indicative for absence of malignant transformation.
BACKGROUND: Oral lichen planus (OLP), a relatively common chronic inflammatory disease of the oral mucosa, is considered to be a premalignant disorder of the oral cavity. Previously, several biomarkers have been tested for their diagnostic potential. Here, we aimed to investigate the diagnostic potential of four miRNAs, miR-21, -125a, -31 and -200a, known to be involved in oral squamous cell carcinoma (OSCC) development, in the saliva of OLP patients as also their putative relation to OSCC development in these patients. MATERIALS AND METHODS: Saliva samples from 30 patients with OLP were collected, 15 of whom were diagnosed with dysplasia upon histopathologic examination. In addition, 15 saliva samples from patients with OSCC and 15 saliva samples from healthy donors were collected. After RNA extraction, the respective miRNA levels were assessed by quantitative RT-PCR. RESULTS: We found that the miR-21 levels were significantly increased in saliva samples derived from patients with OLP, dysplastic OLP and OSCC, compared to those from healthy controls (p = 0.012, p = 0.0017 and p < 0.0001, respectively). Conversely, significant decreases in miR-125a levels were found in the OLP, dysplastic OLP and OSCC samples, compared to those from healthy controls (p < 0.0014, p < 0.0001 and p < 0.0001, respectively). In addition, significant increases in miR-31 levels were found in samples derived from dysplastic OLP and OSCC patients, but not in those from nondysplastic OLP patients, compared to those in healthy controls (p = 0.01 and p = 0.004, respectively). Finally, we found that the miR-200a levels were significantly decreased only in samples derived from OSCC patients (p < 0.0001). CONCLUSIONS: From our data we conclude that increased miR-21 levels in conjunction with decreased miR-125a levels in saliva of OLP patients may be indicative for a poor prognosis. Conversely, we conclude that lack of significant alterations in miR-31 and miR-200a levels in saliva of OLP patients may be indicative for absence of malignant transformation.
Authors: Maria Menini; Emanuele De Giovanni; Francesco Bagnasco; Francesca Delucchi; Francesco Pera; Domenico Baldi; Paolo Pesce Journal: J Pers Med Date: 2021-02-04
Authors: Óscar Rapado-González; Rafael López-López; José Luis López-Cedrún; Gabriel Triana-Martínez; Laura Muinelo-Romay; María Mercedes Suárez-Cunqueiro Journal: Cells Date: 2019-12-17 Impact factor: 6.600