Muqtadir Baig Mirza1, Ayman I Elkady2, Atef M Al-Attar3, Fareeduddin Quadri Syed4, Furkhan Ahmed Mohammed5, Khalid Rehman Hakeem6. 1. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia. Electronic address: mmirza0006@stu.kau.edu.sa. 2. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia; Zoology Department, Faculty of Science, Alexandria University, Alexandria, Egypt. Electronic address: aealkadi@kau.edu.sa. 3. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia. Electronic address: alattar@kau.edu.sa. 4. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia. Electronic address: fsyed@stu.kau.edu.sa. 5. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia. Electronic address: fmohammed0024@stu.kau.edu.sa. 6. Department of Biological Science, Faculty of Science, King Abdulaziz University (KAU), PO Box 80203, Jeddah, Saudi Arabia. Electronic address: khakim@kau.edu.sa.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Phoenix dactylifera L. (Ajwa date) has high nutritive value and are consumed in Arabian Peninsula as an essential diet. Phoenix dactylifera L. have been mentioned in folk remedies of traditional Egyptian medicine and alternative medicine, for numerous health benefits including cancer treatment. The aim of the study is to evaluate the anticancer effects of the extract of Ajwa Date on human Prostate cancer cell line (PC3). MATERIALS AND METHODS: Antiproliferative effect was measured using MTT assay. The long-term effect of EAFAD was determined using colony assay. Different stains like Giemsa and fluorescent stains (DAPI and acridine orange / Ethidium bromide) measured morphological changes. Loss of mitochondrial membrane potential and increased oxidative stress were measured using JC-1 and DCFH-DA dyes. DNA degradation was analyzed by comet assay. Cell cycle distribution was measured by flow cytometer. The apoptotic cell was quantified by annexin V-FITC and Propidium iodide dual staining using flow cytometer. RESULTS: PC3 cell line was treated with ethyl acetate fractions of Ajwa dates (EAFAD) to study their morphological and cellular changes and induction of apoptosis. MTT assay showed the strong inhibitory effect of EAFAD on PC3 cells. Loss of mitochondrial membrane potential and increased oxidative stress were observed in EAFAD treated cells, which suggested mitochondrial involvement in apoptosis. Comet assay proved DNA fragmentation induced by EAFAD. Flow Cytometer results demonstrated that Annexin V-FITC and propidium iodide staining showed that EAFAD induced apoptosis and arrest the cell cycle in S phase. CONCLUSION: Our results suggested EAFAD has potential therapeutics properties for prostate cancer.
ETHNOPHARMACOLOGICAL RELEVANCE: Phoenix dactylifera L. (Ajwa date) has high nutritive value and are consumed in Arabian Peninsula as an essential diet. Phoenix dactylifera L. have been mentioned in folk remedies of traditional Egyptian medicine and alternative medicine, for numerous health benefits including cancer treatment. The aim of the study is to evaluate the anticancer effects of the extract of Ajwa Date on humanProstate cancer cell line (PC3). MATERIALS AND METHODS: Antiproliferative effect was measured using MTT assay. The long-term effect of EAFAD was determined using colony assay. Different stains like Giemsa and fluorescent stains (DAPI and acridine orange / Ethidium bromide) measured morphological changes. Loss of mitochondrial membrane potential and increased oxidative stress were measured using JC-1 and DCFH-DA dyes. DNA degradation was analyzed by comet assay. Cell cycle distribution was measured by flow cytometer. The apoptotic cell was quantified by annexin V-FITC and Propidium iodide dual staining using flow cytometer. RESULTS: PC3 cell line was treated with ethyl acetate fractions of Ajwa dates (EAFAD) to study their morphological and cellular changes and induction of apoptosis. MTT assay showed the strong inhibitory effect of EAFAD on PC3 cells. Loss of mitochondrial membrane potential and increased oxidative stress were observed in EAFAD treated cells, which suggested mitochondrial involvement in apoptosis. Comet assay proved DNA fragmentation induced by EAFAD. Flow Cytometer results demonstrated that Annexin V-FITC and propidium iodide staining showed that EAFAD induced apoptosis and arrest the cell cycle in S phase. CONCLUSION: Our results suggested EAFAD has potential therapeutics properties for prostate cancer.
Authors: Khushboo Shahbaz; Jawaad Ahmed Asif; Tang Liszen; Asma Abdullah Nurul; Mohammad Khursheed Alam Journal: Biomed Res Int Date: 2022-02-07 Impact factor: 3.411