Luisa Zupin 1 , Katia Rupel 1,2 , Giulia Ottaviani 1,2 , Augusto Poropat 1 , Roberto Di Lenarda 1 , Matteo Biasotto 1 , Sergio Crovella 1,3 Show Affiliations »
Abstract
INTRODUCTION: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. OBJECTIVE: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3. MATERIALS AND METHOD: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. RESULTS AND CONCLUSION: In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β -defensins production immediately after treatment, while its efficacy decreases over time. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
INTRODUCTION: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects. OBJECTIVE: This pilot study investigated the effect of LT on the expression of DEFB1 , DEFB4 , DEFB103 genes encoding for the human β-defensins 1, 2 and 3. MATERIALS AND METHOD: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform. RESULTS AND CONCLUSION: In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β -defensins production immediately after treatment, while its efficacy decreases over time. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
Entities: Disease
Gene
Species
Keywords:
Innate immunity; gene expression; laser therapy; oral mucositis; real time PCR; β-defensins.
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Year: 2018
PMID： 29473491 DOI： 10.2174/0929866525666180221123249
Source DB: PubMed Journal: Protein Pept Lett ISSN： 0929-8665 Impact factor: 1.890