| Literature DB >> 29473135 |
Yang Wang1, Chen-Chen Han1, Dongqian Cui1, Ting-Ting Luo1, Yifan Li1, Yuwen Zhang1, Yang Ma2, Wei Wei3.
Abstract
Rheumatoid arthritis (RA) is an autoimmune disease in which T cells play an important role. Paeoniflorin-6-oxy-benzenesulfonate (CP-25) shows a strong anti-inflammatory and immunomodulatory effect in the joint of adjuvant arthritis (AA) rats, but the role of the spleen function is still unclear. The aim of this study was to research how CP-25 regulated spleen function of AA rats. Male Sprague-Dawley rats were administered with CP-25 (50 mg/kg) orally from day 17 to 29 after immunization. The spleen histopathological changes were analyzed by hematoxylin-eosin staining. G protein-coupled receptor kinases (GRKs) and prostaglandin receptor subtypes (EPs) were screened by Western blot and immunohistochemistry. The co-expression of GRK2 and EP2 as well as GRK2 and EP4 was measured by immunofluorescence and co-immunoprecipitation. The expression of GRK2 and EP4 in splenic T cells was further detected by immunofluorescence. CP-25 was found to relieve the secondary paw swelling, attenuate histopathologic changes, and downregulate GRK2, EP2 and EP4 expression in AA rats. Additionally, CP-25 not only downregulated the co-expression of GRK2 and EP4 but also downregulated GRK2, EP4 expression in splenic T cells of AA rats. From these results, we can infer that CP-25 play an anti-inflammatory and immune function by affecting the function of the splenic T cells.Entities:
Keywords: CP-25; G protein-coupled receptor kinases (GRKs); prostaglandin receptor subtypes (EPs); rheumatoid arthritis; spleen
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Year: 2018 PMID： 29473135 DOI： 10.1007/s10753-018-0757-z
Source DB: PubMed Journal: Inflammation ISSN： 0360-3997 Impact factor: 4.092