Complete Genome Sequence of the Facultative Methylotroph Methylobacterium extorquens TK 0001 Isolated from Soil in Poland.

Methylobacterium extorquens TK 0001 (DSM 1337, ATCC 43645) is an aerobic pink-pigmented facultative methylotrophic alphaproteobacterium isolated from soil in Poland. Here, we report the whole-genome sequence and annotation of this organism, which consists of a single 5.71-Mb chromosome.

GENOME ANNOUNCEMENT

The genus Methylobacterium contains rod-shaped Gram-negative bacteria and belongs to the family Rhizobiales. The members of this group of microorganisms are able to grow on reduced C1 compounds such as methane, methanol, and methylamine as sole carbon and energy sources (1, 2). They have been isolated from diverse soil, air, and water environments (3). Methylobacterium extorquens TK 0001 was isolated from soil in Poland (4, 5).

M. extorquens TK 0001 was obtained from the DSMZ (Braunschweig, Germany) and was grown at 30°C in a minimal medium (DSMZ medium no. 1629) supplemented with 1% methanol. Genomic DNA was purified using the GenElute bacterial genomic DNA kit (Sigma). Genome sequencing was performed at Genoscope, using both Illumina and Nanopore technologies (6, 7). First, the long reads generated by the MinION device (Oxford Nanopore Technologies) were corrected using Canu software (8). The corrected reads were assembled using smartdenovo (https://github.com/ruanjue/smartdenovo), and the resulting assembly was then polished with the help of 2 × 150-bp paired-end reads generated by a MiSeq sequencer (Illumina) with a genome coverage of ~134×. The final assembly was composed of a single contig of 5.71 Mb with a GC content of 68.2%. Automatic functional annotation and comparative genome analysis were performed using the MicroScope platform (http://www.genoscope.cns.fr/agc/microscope) (9). In total, 6,251 genomic objects were identified comprising 6,160 coding sequences (CDSs), 17 miscellaneous RNAs, 59 tRNAs, and 15 rRNAs (5 5S, 5 16S, and 5 23S). The M. extorquens TK 0001 genome contains the mxa gene clusters (mxaABCD and mxaFJGIR) coding for methanol dehydrogenase and for enzymes involved in the synthesis of the periplasmic pyrroloquinoline quinone electron carrier. No methylamine dehydrogenase was found. The enzymes for the N-methylglutamate pathway, mgsABC and gmaS, are present, accounting for the growth on methylamine (10). Genes for the serine cycle and the ethylmalonyl-coenzyme A pathway implicated in C1 assimilation are specified. M. extorquens TK 0001 is a facultative methylotroph growing also on complex carbon sources. Accordingly, the Entner-Doudoroff pathway and the pentose phosphate pathway are predicted, as well as the oxidative tricarboxylic acid cycle.

A comparative genome analysis was performed between TK 0001 and M. extorquens strains AM1, PA1, CM4, and DM4 showing that these strains are strongly related. A fraction of 24.3% of the CDSs is specific to M. extorquens strain TK 0001, and 61.9% of the CDSs correspond to the core genome of this group of organisms.

Accession number(s).

This whole-genome project has been deposited in DDBJ/EMBL/GenBank under the accession no. LT962688.