Rupesh Agrawal1,2,3, Sai Bo Bo Tun1, Praveen Kumar Balne1, Hong-Yuan Zhu2, Neha Khandelwal2, Veluchamy A Barathi1,3,4. 1. Translational Preclinical Model Platform, Singapore Eye Research Institute, Singapore. 2. National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore. 3. Yong Loo Lin School of Medicine, National University of Singapore, Singapore. 4. Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School, Singapore.
Abstract
Purpose: To study the effect of anti-VEGF treatment on retinal inflammation in a laser-induced CNV rodent model. Methods: Leukocytes labeled with 1% sodium fluorescein were injected into the laser-induced CNV (wild type C57BL/6) mice at days 4 (baseline), 7, 14, and 19. At baseline intravitreally 3 mice received 1× PBS, and 3 mice received anti-VEGF. FFA, OCT, and SLO were performed at each time point to assess the CNV pathophysiology and inflammatory response. Results: Fluorescein leakage, SRF, and leukocyte infiltration were observed at baseline in both the groups before injection. From days 7 to 19, leukocyte infiltration and SRF were noted in the 1× PBS group, but limited or no SRF and leukocyte infiltration was observed in the anti-VEGF group.Conclusions: Leukocyte infiltration was established as an in vivo imaging inflammatory marker and along with FFA and OCT showed response to anti-VEGF therapy in laser-induced CNV model.
Purpose: To study the effect of anti-VEGF treatment on retinal inflammation in a laser-induced CNV rodent model. Methods: Leukocytes labeled with 1% sodium fluorescein were injected into the laser-induced CNV (wild type C57BL/6) mice at days 4 (baseline), 7, 14, and 19. At baseline intravitreally 3 mice received 1× PBS, and 3 mice received anti-VEGF. FFA, OCT, and SLO were performed at each time point to assess the CNV pathophysiology and inflammatory response. Results:Fluorescein leakage, SRF, and leukocyte infiltration were observed at baseline in both the groups before injection. From days 7 to 19, leukocyte infiltration and SRF were noted in the 1× PBS group, but limited or no SRF and leukocyte infiltration was observed in the anti-VEGF group.Conclusions: Leukocyte infiltration was established as an in vivo imaging inflammatory marker and along with FFA and OCT showed response to anti-VEGF therapy in laser-induced CNV model.
Authors: Waseem Ahamed; Richard Ming Chuan Yu; Yang Pan; Takeshi Iwata; Veluchamy Amutha Barathi; Yeo Sia Wey; Sai Bo Bo Tun; Beiying Qiu; Alison Tan; Xiaomeng Wang; Chui Ming Gemmy Cheung; Tien Yin Wong; Yasuo Yanagi Journal: Int J Mol Sci Date: 2022-09-06 Impact factor: 6.208