Aleksandra Klisic1, Gordana Kocic2, Nebojsa Kavaric3, Milovan Jovanovic3, Verica Stanisic4, Ana Ninic5. 1. Center for Laboratory Diagnostics, Primary Health Care Center, Trg Nikole Kovacevica 6, 81000, Podgorica, Montenegro. aleksandranklisic@gmail.com. 2. Department for Medical Biochemistry, University of Nis-School of Medicine, Nis, Serbia. 3. Center for Laboratory Diagnostics, Primary Health Care Center, Trg Nikole Kovacevica 6, 81000, Podgorica, Montenegro. 4. Clinical Center of Montenegro, Podgorica, Montenegro. 5. Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Abstract
PURPOSE: The pathophysiological mechanism of the relationship between xanthine oxidase (XO) activity and obesity has not been completely elucidated. Since inflammation and oxidative stress are regarded as key determinants of enlarged adipose tissue, we aimed to investigate the association between oxidative stress (as measured with XO activity), inflammation [as measured with high-sensitivity C-reactive protein (hsCRP)] and obesity [as measured with body mass index (BMI)]. In addition, we wanted to examine whether hsCRP itself plays an independent role in XO activity increase or it is only mediated through obesity. METHODS: A total of 118 overweight/obese volunteers (mean age 54.76 ± 15.13 years) were included in the current cross-sectional study. Anthropometric, biochemical parameters, and blood pressure were obtained. RESULTS: Significant differences between age, BMI, waist circumference, concentrations of uric acid and hsCRP, as well as xanthine dehydrogenase (XDH) activities were evident among XO tertile groups. Multiple linear regression analysis revealed that BMI (beta = 0.241, p = 0.012) and XDH (beta = - 0.489, p < 0.001) are the independent predictors of XO activity (R2-adjusted = 0.333), whereas hsCRP lost its independent role in XO activity prediction. CONCLUSION: Obesity (as determined with increased BMI) is an independent predictor of high XO activity in overweight/obese population. LEVEL OF EVIDENCE: Level V: cross-sectional descriptive study.
PURPOSE: The pathophysiological mechanism of the relationship between xanthine oxidase (XO) activity and obesity has not been completely elucidated. Since inflammation and oxidative stress are regarded as key determinants of enlarged adipose tissue, we aimed to investigate the association between oxidative stress (as measured with XO activity), inflammation [as measured with high-sensitivity C-reactive protein (hsCRP)] and obesity [as measured with body mass index (BMI)]. In addition, we wanted to examine whether hsCRP itself plays an independent role in XO activity increase or it is only mediated through obesity. METHODS: A total of 118 overweight/obese volunteers (mean age 54.76 ± 15.13 years) were included in the current cross-sectional study. Anthropometric, biochemical parameters, and blood pressure were obtained. RESULTS: Significant differences between age, BMI, waist circumference, concentrations of uric acid and hsCRP, as well as xanthine dehydrogenase (XDH) activities were evident among XO tertile groups. Multiple linear regression analysis revealed that BMI (beta = 0.241, p = 0.012) and XDH (beta = - 0.489, p < 0.001) are the independent predictors of XO activity (R2-adjusted = 0.333), whereas hsCRP lost its independent role in XO activity prediction. CONCLUSION:Obesity (as determined with increased BMI) is an independent predictor of high XO activity in overweight/obese population. LEVEL OF EVIDENCE: Level V: cross-sectional descriptive study.
Authors: Petar Vukicevic; Aleksandra Klisic; Vojislava Neskovic; Luka Babic; Aleksandar Mikic; Natasa Bogavac-Stanojevic; Milos Matkovic; Vladimir Milićević; Nemanja Aleksic; Jelena Kotur-Stevuljevic Journal: Oxid Med Cell Longev Date: 2021-07-30 Impact factor: 6.543