| Literature DB >> 29468587 |
Mary Susan Lopez1, Raghu Vemuganti2.
Abstract
The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3-7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.Entities:
Keywords: Beam-walk test; Focal cerebral ischemia; Infarct volume; Middle cerebral artery occlusion; Rotarod test; Sticker removal test; Transcardiac perfusion
Mesh：
Year: 2018 PMID： 29468587 DOI： 10.1007/978-1-4939-7526-6_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745