A Rat Model of Hemorrhagic Shock for Studying Vascular Hyperpermeability.

Vascular hyperpermeability is one of the known detrimental effects of hemorrhagic shock, which we continually try to understand, minimize, and reverse. Here, we describe induction of hemorrhagic shock in a rat and studying of its effects on vascular permeability, using intravital microscopy. In this protocol, hemorrhagic shock will be induced by withdrawing blood to reduce the mean arterial pressure (MAP) to 40 mmHg for 60 min followed by resuscitation for 60 min. To study the changes in vascular permeability following hemorrhagic shock, the rats will be given FITC-albumin, a fluorescent tracer, intravenously. Following this, the FITC-albumin flux across the vessel will be measured in mesenteric postcapillary venules by determining fluorescent intensity intravascularly and extravascularly under intravital microscopy.