| Literature DB >> 2945929 |
M Svensson, P Christensen, D Schalén.
Abstract
Spleen cells from mice immunised with group-A type-M15 streptococci and boosted with purified IgG Fc-receptor (FcR) from this type were fused with Sp 2/0 mouse myeloma cells. The resulting hybridomas were screened by ELISA for antibody production. Two IgM-secreting cell lines were selected. The monoclonal antibodies and ascites fluids inhibited the binding of 125I-labelled human IgG and IgG Fc-fragments to group-A type-M15 streptococci. The monoclonal antibodies also displaced purified FcR towards the anode in electrophoresis. They opsonised group-A type M-15 streptococci for phagocytosis by human granulocytes in the presence of fresh human serum. It was concluded that FcR is important for group-A streptococcal virulence.Entities:
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Year: 1986 PMID： 2945929 DOI： 10.1099/00222615-22-3-251
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472