Literature DB >> 2945496

Screening cDNA expression libraries with monoclonal and polyclonal antibodies using an amplified biotin-avidin-peroxidase technique.

B T French, H M Maul, G G Maul.   

Abstract

A chromogenic method using biotinylated secondary antibodies and peroxidase-coupled avidin for screening cDNA expression libraries is described. This method offers increased sensitivity over peroxidase-coupled secondary antibodies and rapid processing of samples, and avoids preparation and handling of radioactive materials. All materials for the chromogenic assay are available commercially and the method offers a fast and easy way to screen lambda and plasmid expression libraries with mono- and polyclonal antibodies.

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Year:  1986        PMID： 2945496     DOI： 10.1016/0003-2697(86)90275-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Cloning, sequencing, expression, and functional studies of a 15,000-molecular-weight Haemophilus somnus antigen similar to Escherichia coli ribosomal protein S9.

Authors:  M Theisen; A A Potter
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

2.  Antikinetochore and antitopoisomerase I antibodies in systemic scleroderma: comparative study using immunoblotted recombinant antigens, immunofluorescence, and double immunodiffusion.

Authors:  M Jarzabek-Chorzelska; M Blaszczyk; Z Kolacinska-Strasz; T Chorzelski; S Jabłońska; G G Maul
Journal:  Arch Dermatol Res       Date:  1990       Impact factor: 3.017

3.  Determination of an epitope of the diffuse systemic sclerosis marker antigen DNA topoisomerase I: sequence similarity with retroviral p30gag protein suggests a possible cause for autoimmunity in systemic sclerosis.

Authors:  G G Maul; S A Jimenez; E Riggs; D Ziemnicka-Kotula
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

4.  Molecular cloning, nucleotide sequence, and characterization of a 40,000-molecular-weight lipoprotein of Haemophilus somnus.

Authors:  M Theisen; C R Rioux; A A Potter
Journal:  Infect Immun       Date:  1992-03       Impact factor: 3.441

5.  Molecular cloning, nucleotide sequence, and characterization of lppB, encoding an antigenic 40-kilodalton lipoprotein of Haemophilus somnus.

Authors:  M Theisen; C R Rioux; A A Potter
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441
  5 in total

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