Yahya Açil1, Mia Leena Arndt2, Aydin Gülses3, Henning Wieker4, Hendrik Naujokat5, Mustafa Ayna6, Jörg Wiltfang7. 1. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: yahya.acil@uksh.de. 2. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: mia-leena@web.de. 3. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: aguelses@mkg.uni-kiel.de. 4. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: henning.wieker@uksh.de. 5. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: naujokat@mkg.uni-kiel.de. 6. Private Practice, Duisburg, Germany. Electronic address: praxis@dr-ayna.de. 7. Christian Albrechts University, Department of Oral and Maxillofacial Surgery, Kiel, Germany. Electronic address: joerg.wiltfang@uksh.de.
Abstract
OBJECTIVE: The aim of this paper was to assess the effects of zoledronate (ZOL) and alendronate (FOS) on apoptotic behavior and gene expression of pro- and inflammatory cytokines of three cell types (human osteoblasts, human gingival fibroblasts and human osteogenic sarcoma cell lines) during a period of 4 weeks. MATERIAL AND METHODS: Cell viability and proliferation was assessed via cell proliferation test (MTT), fluorescence diacetate analysis (FDA). Expression of inflammatory cytokines was investigated by using polymerase chain reaction. RESULTS: The proliferation and cell vitality of osteoblasts and fibroblasts were negatively affected in a dose dependent manner under ZOL and FOS administration. Osteosarcoma cells showed an increase in proliferation under lower doses of BP. ZOL had a significantly higher cytotoxic effect compared with FOS on osteoblasts and fibroblasts. ZOL increased the production of IL-6 in all cell types, whereas FOS only in osteosarcoma cell, which happened in dose dependent manner. Bisphosphonates could result in increase of IL-1β expression of fibroblasts. An increase of IL-12 was observed at higher doses of ZOL administration among osteoblasts and FOS administration in osteosarcoma cells. CONCLUSION: ZOL and FOS could encourage cytotoxic and inflammatory reactions.
OBJECTIVE: The aim of this paper was to assess the effects of zoledronate (ZOL) and alendronate (FOS) on apoptotic behavior and gene expression of pro- and inflammatory cytokines of three cell types (human osteoblasts, human gingival fibroblasts and humanosteogenic sarcoma cell lines) during a period of 4 weeks. MATERIAL AND METHODS: Cell viability and proliferation was assessed via cell proliferation test (MTT), fluorescence diacetate analysis (FDA). Expression of inflammatory cytokines was investigated by using polymerase chain reaction. RESULTS: The proliferation and cell vitality of osteoblasts and fibroblasts were negatively affected in a dose dependent manner under ZOL and FOS administration. Osteosarcoma cells showed an increase in proliferation under lower doses of BP. ZOL had a significantly higher cytotoxic effect compared with FOS on osteoblasts and fibroblasts. ZOL increased the production of IL-6 in all cell types, whereas FOS only in osteosarcoma cell, which happened in dose dependent manner. Bisphosphonates could result in increase of IL-1β expression of fibroblasts. An increase of IL-12 was observed at higher doses of ZOL administration among osteoblasts and FOS administration in osteosarcoma cells. CONCLUSION:ZOL and FOS could encourage cytotoxic and inflammatory reactions.
Authors: Sheng Zhu; Victor Häussling; Romina H Aspera-Werz; Tao Chen; Bianca Braun; Weidong Weng; Tina Histing; Andreas K Nussler Journal: Int J Mol Sci Date: 2020-12-23 Impact factor: 5.923
Authors: David André Barrière; Élora Midavaine; Louis Doré-Savard; Karyn Kirby; Luc Tremblay; Jean-François Beaudoin; Nicolas Beaudet; Jean-Michel Longpré; Roger Lecomte; Martin Lepage; Philippe Sarret Journal: Sci Rep Date: 2019-12-27 Impact factor: 4.379