| Literature DB >> 29453567 |
Marina G Yefimova1,2, Nadia Messaddeq3,4,5,6, Annie-Claire Meunier1, Anne Cantereau1,7, Bernard Jegou8, Nicolas Bourmeyster9,10.
Abstract
Sertoli cells were discovered in the seminiferous tubules by Enrico Sertoli in 1865 (Morgagni 7:31-33, 1865). Intense phagocytosis is, in the context of spermatogenesis cycle, morphologically the most noticeable function of Sertoli cells. In this chapter the major principles of phagocytosis machinery and its specificities in the seminiferous tubules will be briefly reviewed, guidelines of analysis of main phagocytosis steps by confocal and transmission electron microscopy will be described, and a simplified method to assess phagocytosis rate in routine experiments will be given.Keywords: Apoptotic-like membranes; Homeostatic phagocytosis; Nonprofessional phagocytes; Phagocytosis steps; Residual bodies; Sertoli cells
Mesh：
Year: 2018 PMID： 29453567 DOI： 10.1007/978-1-4939-7698-0_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745